Tensile strength of human pericardium treated with glutaraldehyde

Hiromasa Yamashita, Shigeyuki Ozaki, Kiyotaka Iwasaki, Isamu Kawase, Yukinari Nozawa, Mitsuo Umezu

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Purpose: We have reconstructed aortic valves using autologous pericardium treated with glutaraldehyde since April 2007. However, the strength of the human pericardium has not been confirmed. We compared tensile strength between glutaraldehyde-treated human pericardium and aortic valve leaflets with various degrees of calcification to determine their suitability for use in aortic valve reconstruction. Methods: We measured the ultimate tensile strength and elasticity of samples of glutaraldehyde- treated pericardia (n = 8), non-calcified (n = 12), calcified (n = 9) and decalcified (n = 21) aortic leaflets collected from 23 patients who underwent aortic valve surgery. Aortic valves were decalcified using a cavitational ultrasonic surgical aspirator. The pericardium was immersed in 0.6% buffered glutaraldehyde for 10 minutes and then rinsed three times for 6 minutes each in normal saline. Results: The ultimate tensile strength of the glutaraldehyde-treated human pericardium, non-calcified, calcified and decalcified leaflets was 10, 2.8, 1.0 and 0.8 MPa, respectively. Conclusions: The ultimate tensile strength of glutaraldehyde-treated human pericardium was 4 times higher than non-calcified leaflets, indicating its suitability for application to aortic valve reconstruction. Calcified leaflets were slightly stronger than decalcified leaflets. Thus, calcification can be removed without altering the tensile strength of valve materials.

    Original languageEnglish
    Pages (from-to)434-437
    Number of pages4
    JournalAnnals of Thoracic and Cardiovascular Surgery
    Volume18
    Issue number5
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Tensile Strength
    Pericardium
    Glutaral
    Aortic Valve
    Elasticity
    Ultrasonics

    Keywords

    • Aortic valve reconstruction
    • Autologous pericardium
    • Glutaraldehyde treatment
    • Mechanical strength

    ASJC Scopus subject areas

    • Surgery
    • Cardiology and Cardiovascular Medicine
    • Pulmonary and Respiratory Medicine
    • Gastroenterology

    Cite this

    Tensile strength of human pericardium treated with glutaraldehyde. / Yamashita, Hiromasa; Ozaki, Shigeyuki; Iwasaki, Kiyotaka; Kawase, Isamu; Nozawa, Yukinari; Umezu, Mitsuo.

    In: Annals of Thoracic and Cardiovascular Surgery, Vol. 18, No. 5, 2012, p. 434-437.

    Research output: Contribution to journalArticle

    Yamashita, Hiromasa ; Ozaki, Shigeyuki ; Iwasaki, Kiyotaka ; Kawase, Isamu ; Nozawa, Yukinari ; Umezu, Mitsuo. / Tensile strength of human pericardium treated with glutaraldehyde. In: Annals of Thoracic and Cardiovascular Surgery. 2012 ; Vol. 18, No. 5. pp. 434-437.
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    abstract = "Purpose: We have reconstructed aortic valves using autologous pericardium treated with glutaraldehyde since April 2007. However, the strength of the human pericardium has not been confirmed. We compared tensile strength between glutaraldehyde-treated human pericardium and aortic valve leaflets with various degrees of calcification to determine their suitability for use in aortic valve reconstruction. Methods: We measured the ultimate tensile strength and elasticity of samples of glutaraldehyde- treated pericardia (n = 8), non-calcified (n = 12), calcified (n = 9) and decalcified (n = 21) aortic leaflets collected from 23 patients who underwent aortic valve surgery. Aortic valves were decalcified using a cavitational ultrasonic surgical aspirator. The pericardium was immersed in 0.6{\%} buffered glutaraldehyde for 10 minutes and then rinsed three times for 6 minutes each in normal saline. Results: The ultimate tensile strength of the glutaraldehyde-treated human pericardium, non-calcified, calcified and decalcified leaflets was 10, 2.8, 1.0 and 0.8 MPa, respectively. Conclusions: The ultimate tensile strength of glutaraldehyde-treated human pericardium was 4 times higher than non-calcified leaflets, indicating its suitability for application to aortic valve reconstruction. Calcified leaflets were slightly stronger than decalcified leaflets. Thus, calcification can be removed without altering the tensile strength of valve materials.",
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