Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions

Mio Takeuchi*, Takao Yamagishi, Yoichi Kamagata, Kenshiro Oshima, Masahira Hattori, Taiki Katayama, Satoshi Hanada, Hideyuki Tamaki, Katsumi Marumo, Hiroto Maeda, Munetomo Nedachi, Wataru Iwasaki, Yuichi Suwa, Susumu Sakata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A moderately thermophilic, aerobic, stalked bacterium (strain MA2<sup>T</sup>) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2<sup>T</sup>was most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92–93% similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16– 48 ºC (optimum growth at 42 ºC). This strain required yeast extract and NaCl (>1%, w/v) for growth, tolerated up to 11% (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C<inf>18: 1</inf>ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2<sup>T</sup>performed denitrification and produced N<inf>2</inf>O from nitrate under strictly microaerobic conditions. Strain MA2<sup>T</sup>possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2<sup>T</sup>(=NBRC 109643<sup>T</sup>=DSM 27167<sup>T</sup>) as the type strain of the species.

Original languageEnglish
Pages (from-to)1749-1754
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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