Tertiary structural analysis of Escherichia coli lysine tRNA.

N. Hayashi, G. Kawai, M. Takayanagi, T. Noguchi, Takuya Ueda, K. Nishikawa, K. Miura, T. Miyazawa, S. Yokoyama, K. Watanabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

It has been suggested that Escherichia coli lysine tRNA (E. coli tRNA(Lys)) has an unusual anticodon loop structure, judging from the abnormal CD pattern derived from 5-methylaminomethyl-2-thiouridine (mnm5s2U) located at the first position of the anticodon of the tRNA. Here, the structure of E. coli tRNA(Lys) was analysed by chemical modification and nuclear magnetic resonance (NMR) of the imino protons. The chemical probing approach showed that the anticodon arm region forms the ordinary stem-and-loop structure as expected from the standard clover-leaf structure, because N7 of G30, N3 of C40, N7 of A41 and N7 of A42 were protected under a native condition. By using the heteronuclear 15N-1H single quantum coherence (HSQC) spectra of 15N-labeled E.coli tRNA(Lys) and the analyses of the nuclear Overhauser effects (NOE) between the imino protons of non labeled E.coli tRNA(Lys), the imino protons in the acceptor stem was assigned, which indicates that the acceptor stem exists in an usual form. The tertiary base-base interactions was also detected. Thus, E.coli tRNA(Lys) seems to maintain a similar structure to the ordinary L-shaped tertiary structure, as a whole. The fine structure of the anticodon loop is now being analyzed.

Original languageEnglish
Pages (from-to)195-196
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993 Jan 1
Externally publishedYes

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RNA, Transfer, Lys
Transfer RNA
Lysine
Anticodon
Escherichia coli
Protons
Medicago
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hayashi, N., Kawai, G., Takayanagi, M., Noguchi, T., Ueda, T., Nishikawa, K., ... Watanabe, K. (1993). Tertiary structural analysis of Escherichia coli lysine tRNA. Nucleic acids symposium series, (29), 195-196.

Tertiary structural analysis of Escherichia coli lysine tRNA. / Hayashi, N.; Kawai, G.; Takayanagi, M.; Noguchi, T.; Ueda, Takuya; Nishikawa, K.; Miura, K.; Miyazawa, T.; Yokoyama, S.; Watanabe, K.

In: Nucleic acids symposium series, No. 29, 01.01.1993, p. 195-196.

Research output: Contribution to journalArticle

Hayashi, N, Kawai, G, Takayanagi, M, Noguchi, T, Ueda, T, Nishikawa, K, Miura, K, Miyazawa, T, Yokoyama, S & Watanabe, K 1993, 'Tertiary structural analysis of Escherichia coli lysine tRNA.', Nucleic acids symposium series, no. 29, pp. 195-196.
Hayashi N, Kawai G, Takayanagi M, Noguchi T, Ueda T, Nishikawa K et al. Tertiary structural analysis of Escherichia coli lysine tRNA. Nucleic acids symposium series. 1993 Jan 1;(29):195-196.
Hayashi, N. ; Kawai, G. ; Takayanagi, M. ; Noguchi, T. ; Ueda, Takuya ; Nishikawa, K. ; Miura, K. ; Miyazawa, T. ; Yokoyama, S. ; Watanabe, K. / Tertiary structural analysis of Escherichia coli lysine tRNA. In: Nucleic acids symposium series. 1993 ; No. 29. pp. 195-196.
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