Tertiary structures of mitochondrial tRNAs having characteristic secondary structures.

Y. Watanabe, G. Kawai, T. Yokogawa, I. Hayashi, N. Hayashi, K. Nishikawa, Takuya Ueda, K. Watanabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Some of the animal mitochondrial (mt) tRNAs are thought to have unusual secondary structures as inferred from the mt DNA sequence analysis. To obtain information on the relationship between the unusual secondary and tertiary structures and function of these mt tRNAs at the RNA level, we have analyzed the structure of bovine mt serine tRNAs and obtained the following results. 1) Using the novel secondary structure proposed on the basis of enzymatic probing and phylogenetic comparison and the known tertiary structure of yeast tRNA(Phe), a tertiary structural model of tRNA(Ser) specific for UCU/C/A/G codons was constructed by a computer modeling. In the proposed model, one-base-pair elongation of anticodon stem compensated for the deletions in some of the loop regions of this tRNA, and the model maintained the topological relationship between the anticodon and 3'-CCA terminus as nearly the same as that of yeast tRNA(Phe). The results of chemical modification of this tRNA supported the proposed model. 2) For NMR analysis of tRNA(Ser) specific for AGU/C codons and lacking the D stem, a large scale preparation of the unmodified tRNA transcript has been performed by the use of an in vitro T7 transcription system.

Original languageEnglish
Pages (from-to)209-210
Number of pages2
JournalNucleic acids symposium series
Issue number29
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Fingerprint

Transfer RNA
RNA, Transfer, Ser
RNA, Transfer, Phe
Anticodon
Codon
Yeasts
Structural Models
Mitochondrial DNA
DNA Sequence Analysis
Base Pairing
Serine
RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Watanabe, Y., Kawai, G., Yokogawa, T., Hayashi, I., Hayashi, N., Nishikawa, K., ... Watanabe, K. (1993). Tertiary structures of mitochondrial tRNAs having characteristic secondary structures. Nucleic acids symposium series, (29), 209-210.

Tertiary structures of mitochondrial tRNAs having characteristic secondary structures. / Watanabe, Y.; Kawai, G.; Yokogawa, T.; Hayashi, I.; Hayashi, N.; Nishikawa, K.; Ueda, Takuya; Watanabe, K.

In: Nucleic acids symposium series, No. 29, 01.01.1993, p. 209-210.

Research output: Contribution to journalArticle

Watanabe, Y, Kawai, G, Yokogawa, T, Hayashi, I, Hayashi, N, Nishikawa, K, Ueda, T & Watanabe, K 1993, 'Tertiary structures of mitochondrial tRNAs having characteristic secondary structures.', Nucleic acids symposium series, no. 29, pp. 209-210.
Watanabe Y, Kawai G, Yokogawa T, Hayashi I, Hayashi N, Nishikawa K et al. Tertiary structures of mitochondrial tRNAs having characteristic secondary structures. Nucleic acids symposium series. 1993 Jan 1;(29):209-210.
Watanabe, Y. ; Kawai, G. ; Yokogawa, T. ; Hayashi, I. ; Hayashi, N. ; Nishikawa, K. ; Ueda, Takuya ; Watanabe, K. / Tertiary structures of mitochondrial tRNAs having characteristic secondary structures. In: Nucleic acids symposium series. 1993 ; No. 29. pp. 209-210.
@article{7ccdeca103304e59a652ea6ce0781d8c,
title = "Tertiary structures of mitochondrial tRNAs having characteristic secondary structures.",
abstract = "Some of the animal mitochondrial (mt) tRNAs are thought to have unusual secondary structures as inferred from the mt DNA sequence analysis. To obtain information on the relationship between the unusual secondary and tertiary structures and function of these mt tRNAs at the RNA level, we have analyzed the structure of bovine mt serine tRNAs and obtained the following results. 1) Using the novel secondary structure proposed on the basis of enzymatic probing and phylogenetic comparison and the known tertiary structure of yeast tRNA(Phe), a tertiary structural model of tRNA(Ser) specific for UCU/C/A/G codons was constructed by a computer modeling. In the proposed model, one-base-pair elongation of anticodon stem compensated for the deletions in some of the loop regions of this tRNA, and the model maintained the topological relationship between the anticodon and 3'-CCA terminus as nearly the same as that of yeast tRNA(Phe). The results of chemical modification of this tRNA supported the proposed model. 2) For NMR analysis of tRNA(Ser) specific for AGU/C codons and lacking the D stem, a large scale preparation of the unmodified tRNA transcript has been performed by the use of an in vitro T7 transcription system.",
author = "Y. Watanabe and G. Kawai and T. Yokogawa and I. Hayashi and N. Hayashi and K. Nishikawa and Takuya Ueda and K. Watanabe",
year = "1993",
month = "1",
day = "1",
language = "English",
pages = "209--210",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "29",

}

TY - JOUR

T1 - Tertiary structures of mitochondrial tRNAs having characteristic secondary structures.

AU - Watanabe, Y.

AU - Kawai, G.

AU - Yokogawa, T.

AU - Hayashi, I.

AU - Hayashi, N.

AU - Nishikawa, K.

AU - Ueda, Takuya

AU - Watanabe, K.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Some of the animal mitochondrial (mt) tRNAs are thought to have unusual secondary structures as inferred from the mt DNA sequence analysis. To obtain information on the relationship between the unusual secondary and tertiary structures and function of these mt tRNAs at the RNA level, we have analyzed the structure of bovine mt serine tRNAs and obtained the following results. 1) Using the novel secondary structure proposed on the basis of enzymatic probing and phylogenetic comparison and the known tertiary structure of yeast tRNA(Phe), a tertiary structural model of tRNA(Ser) specific for UCU/C/A/G codons was constructed by a computer modeling. In the proposed model, one-base-pair elongation of anticodon stem compensated for the deletions in some of the loop regions of this tRNA, and the model maintained the topological relationship between the anticodon and 3'-CCA terminus as nearly the same as that of yeast tRNA(Phe). The results of chemical modification of this tRNA supported the proposed model. 2) For NMR analysis of tRNA(Ser) specific for AGU/C codons and lacking the D stem, a large scale preparation of the unmodified tRNA transcript has been performed by the use of an in vitro T7 transcription system.

AB - Some of the animal mitochondrial (mt) tRNAs are thought to have unusual secondary structures as inferred from the mt DNA sequence analysis. To obtain information on the relationship between the unusual secondary and tertiary structures and function of these mt tRNAs at the RNA level, we have analyzed the structure of bovine mt serine tRNAs and obtained the following results. 1) Using the novel secondary structure proposed on the basis of enzymatic probing and phylogenetic comparison and the known tertiary structure of yeast tRNA(Phe), a tertiary structural model of tRNA(Ser) specific for UCU/C/A/G codons was constructed by a computer modeling. In the proposed model, one-base-pair elongation of anticodon stem compensated for the deletions in some of the loop regions of this tRNA, and the model maintained the topological relationship between the anticodon and 3'-CCA terminus as nearly the same as that of yeast tRNA(Phe). The results of chemical modification of this tRNA supported the proposed model. 2) For NMR analysis of tRNA(Ser) specific for AGU/C codons and lacking the D stem, a large scale preparation of the unmodified tRNA transcript has been performed by the use of an in vitro T7 transcription system.

UR - http://www.scopus.com/inward/record.url?scp=0027448468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027448468&partnerID=8YFLogxK

M3 - Article

SP - 209

EP - 210

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 29

ER -