Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada & 4 others Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalCell Reports
Volume18
Issue number3
DOIs
Publication statusPublished - 2017 Jan 17

Fingerprint

Nucleosomes
Essential Genes
Spermatogenesis
Germ Cells
Histones
Testis
Genes
Cells
Azoospermia
Spermatogonia
Chromatin Assembly and Disassembly
Haploidy
Meiosis
Epigenomics
Chromatin
Genome
Proteins

Keywords

  • chromatin
  • crystal structure
  • epigenetics
  • histone variant
  • meiosis
  • nucleosome
  • spermatogenesis
  • spermatogonial differentiation
  • spermatozoa
  • testes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ueda, J., Harada, A., Urahama, T., Machida, S., Maehara, K., Hada, M., ... Yamagata, K. (2017). Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. Cell Reports, 18(3), 593-600. https://doi.org/10.1016/j.celrep.2016.12.065

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. / Ueda, Jun; Harada, Akihito; Urahama, Takashi; Machida, Shinichi; Maehara, Kazumitsu; Hada, Masashi; Makino, Yoshinori; Nogami, Jumpei; Horikoshi, Naoki; Osakabe, Akihisa; Taguchi, Hiroyuki; Tanaka, Hiroki; Tachiwana, Hiroaki; Yao, Tatsuma; Yamada, Minami; Iwamoto, Takashi; Isotani, Ayako; Ikawa, Masahito; Tachibana, Taro; Okada, Yuki; Kimura, Hiroshi; Ohkawa, Yasuyuki; Kurumizaka, Hitoshi; Yamagata, Kazuo.

In: Cell Reports, Vol. 18, No. 3, 17.01.2017, p. 593-600.

Research output: Contribution to journalArticle

Ueda, J, Harada, A, Urahama, T, Machida, S, Maehara, K, Hada, M, Makino, Y, Nogami, J, Horikoshi, N, Osakabe, A, Taguchi, H, Tanaka, H, Tachiwana, H, Yao, T, Yamada, M, Iwamoto, T, Isotani, A, Ikawa, M, Tachibana, T, Okada, Y, Kimura, H, Ohkawa, Y, Kurumizaka, H & Yamagata, K 2017, 'Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis', Cell Reports, vol. 18, no. 3, pp. 593-600. https://doi.org/10.1016/j.celrep.2016.12.065
Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M et al. Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. Cell Reports. 2017 Jan 17;18(3):593-600. https://doi.org/10.1016/j.celrep.2016.12.065
Ueda, Jun ; Harada, Akihito ; Urahama, Takashi ; Machida, Shinichi ; Maehara, Kazumitsu ; Hada, Masashi ; Makino, Yoshinori ; Nogami, Jumpei ; Horikoshi, Naoki ; Osakabe, Akihisa ; Taguchi, Hiroyuki ; Tanaka, Hiroki ; Tachiwana, Hiroaki ; Yao, Tatsuma ; Yamada, Minami ; Iwamoto, Takashi ; Isotani, Ayako ; Ikawa, Masahito ; Tachibana, Taro ; Okada, Yuki ; Kimura, Hiroshi ; Ohkawa, Yasuyuki ; Kurumizaka, Hitoshi ; Yamagata, Kazuo. / Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. In: Cell Reports. 2017 ; Vol. 18, No. 3. pp. 593-600.
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