Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells

Koji Atarashi, Takeshi Tanoue, Minoru Ando, Nobuhiko Kamada, Yuji Nagano, Seiko Narushima, Wataru Suda, Akemi Imaoka, Hiromi Setoyama, Takashi Nagamori, Eiji Ishikawa, Tatsuichiro Shima, Taeko Hara, Shoichi Kado, Toshi Jinnohara, Hiroshi Ohno, Takashi Kondo, Kiminori Toyooka, Eiichiro Watanabe, Shin Ichiro Yokoyama & 11 others Shunji Tokoro, Hiroshi Mori, Yurika Noguchi, Hidetoshi Morita, Ivaylo I. Ivanov, Tsuyoshi Sugiyama, Gabriel Nuñez, J. Gray Camp, Masahira Hattori, Yoshinori Umesaki, Kenya Honda

Research output: Contribution to journalArticle

295 Citations (Scopus)

Abstract

Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.

Original languageEnglish
Pages (from-to)367-380
Number of pages14
JournalCell
Volume163
Issue number2
DOIs
Publication statusPublished - 2015 Oct 8
Externally publishedYes

Fingerprint

Th17 Cells
Cell Adhesion
Bacteria
Adhesion
Epithelial Cells
Rats
Citrobacter rodentium
Aptitude
Escherichia coli O157
Pathogens
Ulcerative Colitis
Adhesives
Escherichia coli
Cues
Colon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Atarashi, K., Tanoue, T., Ando, M., Kamada, N., Nagano, Y., Narushima, S., ... Honda, K. (2015). Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells. Cell, 163(2), 367-380. https://doi.org/10.1016/j.cell.2015.08.058

Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells. / Atarashi, Koji; Tanoue, Takeshi; Ando, Minoru; Kamada, Nobuhiko; Nagano, Yuji; Narushima, Seiko; Suda, Wataru; Imaoka, Akemi; Setoyama, Hiromi; Nagamori, Takashi; Ishikawa, Eiji; Shima, Tatsuichiro; Hara, Taeko; Kado, Shoichi; Jinnohara, Toshi; Ohno, Hiroshi; Kondo, Takashi; Toyooka, Kiminori; Watanabe, Eiichiro; Yokoyama, Shin Ichiro; Tokoro, Shunji; Mori, Hiroshi; Noguchi, Yurika; Morita, Hidetoshi; Ivanov, Ivaylo I.; Sugiyama, Tsuyoshi; Nuñez, Gabriel; Camp, J. Gray; Hattori, Masahira; Umesaki, Yoshinori; Honda, Kenya.

In: Cell, Vol. 163, No. 2, 08.10.2015, p. 367-380.

Research output: Contribution to journalArticle

Atarashi, K, Tanoue, T, Ando, M, Kamada, N, Nagano, Y, Narushima, S, Suda, W, Imaoka, A, Setoyama, H, Nagamori, T, Ishikawa, E, Shima, T, Hara, T, Kado, S, Jinnohara, T, Ohno, H, Kondo, T, Toyooka, K, Watanabe, E, Yokoyama, SI, Tokoro, S, Mori, H, Noguchi, Y, Morita, H, Ivanov, II, Sugiyama, T, Nuñez, G, Camp, JG, Hattori, M, Umesaki, Y & Honda, K 2015, 'Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells', Cell, vol. 163, no. 2, pp. 367-380. https://doi.org/10.1016/j.cell.2015.08.058
Atarashi K, Tanoue T, Ando M, Kamada N, Nagano Y, Narushima S et al. Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells. Cell. 2015 Oct 8;163(2):367-380. https://doi.org/10.1016/j.cell.2015.08.058
Atarashi, Koji ; Tanoue, Takeshi ; Ando, Minoru ; Kamada, Nobuhiko ; Nagano, Yuji ; Narushima, Seiko ; Suda, Wataru ; Imaoka, Akemi ; Setoyama, Hiromi ; Nagamori, Takashi ; Ishikawa, Eiji ; Shima, Tatsuichiro ; Hara, Taeko ; Kado, Shoichi ; Jinnohara, Toshi ; Ohno, Hiroshi ; Kondo, Takashi ; Toyooka, Kiminori ; Watanabe, Eiichiro ; Yokoyama, Shin Ichiro ; Tokoro, Shunji ; Mori, Hiroshi ; Noguchi, Yurika ; Morita, Hidetoshi ; Ivanov, Ivaylo I. ; Sugiyama, Tsuyoshi ; Nuñez, Gabriel ; Camp, J. Gray ; Hattori, Masahira ; Umesaki, Yoshinori ; Honda, Kenya. / Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells. In: Cell. 2015 ; Vol. 163, No. 2. pp. 367-380.
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