The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment

Naoki Aoyama, Asako Oka, Kumiko Kitayama, Hitoshi Kurumizaka, Masahiko Harata

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The actin family consists of conventional actin and various actin-related proteins (Arps). Some of these Arps are localized in the nucleus, and a fraction of each of these nuclear Arps is functionally involved in chromatin remodeling and histone acetyltransferase complexes. On the other hand, in mitotic cells, the localization and function of the nuclear Arps are largely unknown. Human Arp8 (hArp8), an ortholog of yeast nuclear Arp8, was recently found to be associated with the hINO80-chromatin remodeling complex along with hArp5. Here we report that hArp8, but not hArp5, accumulates on mitotic chromosomes. This is the first example where a member of the actin family is found to be associated with mitotic chromosomes. Expression of truncated hArp8 proteins and depletion of endogenous hArp8 by RNA interference caused misalignment of mitotic chromosomes, suggesting that chromosome-associated hArp8 has a role in chromosome behavior. In contrast, depletion of hIno80 and hArp5 did not cause misalignment of chromosomes, suggesting that the role of hArp8 at mitotic chromosomes is independent of the activity of hINO80 complexes. These findings provide the first insight into a novel function of actin family members in mitosis.

    Original languageEnglish
    Pages (from-to)859-868
    Number of pages10
    JournalExperimental Cell Research
    Volume314
    Issue number4
    DOIs
    Publication statusPublished - 2008 Feb 15

    Fingerprint

    Actins
    Chromosomes
    Chromatin Assembly and Disassembly
    Proteins
    Histone Acetyltransferases
    human ACTR8 protein
    Human Chromosomes
    RNA Interference
    Mitosis
    Yeasts

    Keywords

    • Actin family
    • Actin-related protein
    • Cell nucleus
    • Chromatin remodeling complex
    • Mitosis
    • Mitotic chromosome

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment. / Aoyama, Naoki; Oka, Asako; Kitayama, Kumiko; Kurumizaka, Hitoshi; Harata, Masahiko.

    In: Experimental Cell Research, Vol. 314, No. 4, 15.02.2008, p. 859-868.

    Research output: Contribution to journalArticle

    Aoyama, Naoki ; Oka, Asako ; Kitayama, Kumiko ; Kurumizaka, Hitoshi ; Harata, Masahiko. / The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment. In: Experimental Cell Research. 2008 ; Vol. 314, No. 4. pp. 859-868.
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