The alpha-subunit of glycoprotein hormones exists in the prolactin secretory granules of the bullforg (Rana catesbeiana) pituitary gland

Shigeyasu Tanaka, Fumihiko Mizutani, Kazutoshi Yamamoto, Sakaé Kikuyama, Kazumasa Kurosumi

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Our recent finding that the number of immunoreactive α-subunit cells was invariably greater than the total number of immunoreactive gonadotropin (GTH) and thyrotropin (TSH) cells in the bullfrog (Rana catesbeiana) pituitary gland raises the possibility that the α-subunit also exists in pituitary cells other than GTH and TSH cells. The present study demonstrates that there are a considerable number of immunoreactive prolactin (PRL) cells that are also stained with antibody against the α-subunit when adjacent sections are immunocytochemically examined. Neither immunoreactive growth hormone nor adrenocorticotropin cells are stained with the antibody against the α-subunit. The specificity of the antibody against the α-subunit and of that against PRL was demonstrated by preabsorption test, non-competitive binding test, and immunoblot analysis. Double-immunolabeling with gold particles of different sizes for the α-subunit and PRL revealed that most of the immunolabeled PRL-secretory granules are also labeled with the α-subunit antibody. The gold particles indicating the presence of the α-subunit were mostly found in the peripheral zone of the secretory granules.

    Original languageEnglish
    Pages (from-to)223-231
    Number of pages9
    JournalCell and Tissue Research
    Volume267
    Issue number2
    DOIs
    Publication statusPublished - 1992 Feb

    Fingerprint

    Alpha Subunit Glycoprotein Hormones
    Rana catesbeiana
    Secretory Vesicles
    Pituitary Gland
    Prolactin
    Thyrotrophs
    Antibodies
    Gold
    Antibody Specificity
    Thyrotropin
    Gonadotropins
    Particle Size
    Adrenocorticotropic Hormone
    Growth Hormone

    Keywords

    • α-Subunit
    • Bullfrog, Rana catesbeiana (Anura)
    • Colocalization
    • Immunocytochemistry
    • Pars distalis
    • Pituitary glycoprotein hormone
    • PRL cell

    ASJC Scopus subject areas

    • Anatomy
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    The alpha-subunit of glycoprotein hormones exists in the prolactin secretory granules of the bullforg (Rana catesbeiana) pituitary gland. / Tanaka, Shigeyasu; Mizutani, Fumihiko; Yamamoto, Kazutoshi; Kikuyama, Sakaé; Kurosumi, Kazumasa.

    In: Cell and Tissue Research, Vol. 267, No. 2, 02.1992, p. 223-231.

    Research output: Contribution to journalArticle

    Tanaka, Shigeyasu ; Mizutani, Fumihiko ; Yamamoto, Kazutoshi ; Kikuyama, Sakaé ; Kurosumi, Kazumasa. / The alpha-subunit of glycoprotein hormones exists in the prolactin secretory granules of the bullforg (Rana catesbeiana) pituitary gland. In: Cell and Tissue Research. 1992 ; Vol. 267, No. 2. pp. 223-231.
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