The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance

Akane G. Mizukami, Rie Inatsugi, Jiao Jiao, Toshihisa Kotake, Keiko Kuwata, Kento Ootani, Satohiro Okuda, Subramanian Sankaranarayanan, Yoshikatsu Sato, Daisuke Maruyama, Hiroaki Iwai, Estelle Garénaux, Chihiro Sato, Ken Kitajima, Yoichi Tsumuraya, Hitoshi Mori, Junichiro Yamaguchi, Kenichiro Itami, Narie Sasaki, Tetsuya Higashiyama

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [1-3]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [4-6]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [1, 3, 7]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

Original languageEnglish
Pages (from-to)1091-1097
Number of pages7
JournalCurrent Biology
Volume26
Issue number8
DOIs
Publication statusPublished - 2016 Apr 25
Externally publishedYes

Fingerprint

Pollen Tube
arabinogalactans
pollen tubes
Sugars
sugars
Peptides
Ovule
Molecules
Disaccharides
pistil
peptides
attractants
Galactose
ovules
Isomers
Polysaccharides
Torenia
Torenia fournieri
Tissue
Plant Structures

Keywords

  • arabinogalactan
  • competency control
  • LURE attractant peptides
  • ovule
  • pollen-tube guidance
  • Torenia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mizukami, A. G., Inatsugi, R., Jiao, J., Kotake, T., Kuwata, K., Ootani, K., ... Higashiyama, T. (2016). The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Current Biology, 26(8), 1091-1097. https://doi.org/10.1016/j.cub.2016.02.040

The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. / Mizukami, Akane G.; Inatsugi, Rie; Jiao, Jiao; Kotake, Toshihisa; Kuwata, Keiko; Ootani, Kento; Okuda, Satohiro; Sankaranarayanan, Subramanian; Sato, Yoshikatsu; Maruyama, Daisuke; Iwai, Hiroaki; Garénaux, Estelle; Sato, Chihiro; Kitajima, Ken; Tsumuraya, Yoichi; Mori, Hitoshi; Yamaguchi, Junichiro; Itami, Kenichiro; Sasaki, Narie; Higashiyama, Tetsuya.

In: Current Biology, Vol. 26, No. 8, 25.04.2016, p. 1091-1097.

Research output: Contribution to journalArticle

Mizukami, AG, Inatsugi, R, Jiao, J, Kotake, T, Kuwata, K, Ootani, K, Okuda, S, Sankaranarayanan, S, Sato, Y, Maruyama, D, Iwai, H, Garénaux, E, Sato, C, Kitajima, K, Tsumuraya, Y, Mori, H, Yamaguchi, J, Itami, K, Sasaki, N & Higashiyama, T 2016, 'The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance', Current Biology, vol. 26, no. 8, pp. 1091-1097. https://doi.org/10.1016/j.cub.2016.02.040
Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K et al. The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. Current Biology. 2016 Apr 25;26(8):1091-1097. https://doi.org/10.1016/j.cub.2016.02.040
Mizukami, Akane G. ; Inatsugi, Rie ; Jiao, Jiao ; Kotake, Toshihisa ; Kuwata, Keiko ; Ootani, Kento ; Okuda, Satohiro ; Sankaranarayanan, Subramanian ; Sato, Yoshikatsu ; Maruyama, Daisuke ; Iwai, Hiroaki ; Garénaux, Estelle ; Sato, Chihiro ; Kitajima, Ken ; Tsumuraya, Yoichi ; Mori, Hitoshi ; Yamaguchi, Junichiro ; Itami, Kenichiro ; Sasaki, Narie ; Higashiyama, Tetsuya. / The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. In: Current Biology. 2016 ; Vol. 26, No. 8. pp. 1091-1097.
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