The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly

Kishiko Ogawa, Katsuhiko Suzuki, Okutsu Mitsuharu, Kyoko Yamazaki, Shoji Shinkai

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.

Original languageEnglish
Article number13
JournalImmunity and Ageing
Volume5
DOIs
Publication statusPublished - 2008 Oct 24

Fingerprint

Reactive Oxygen Species
Neutrophils
Inflammation
N-Formylmethionine Leucyl-Phenylalanine
Serum Amyloid A Protein
HSP70 Heat-Shock Proteins
Chemokine CCL2
Peroxides
Interleukin-8
Interleukin-1
C-Reactive Protein
Free Radicals
Lipopolysaccharides
Interleukin-6
Flow Cytometry
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Ions
Cytokines
Oxygen

ASJC Scopus subject areas

  • Immunology
  • Ageing

Cite this

The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly. / Ogawa, Kishiko; Suzuki, Katsuhiko; Mitsuharu, Okutsu; Yamazaki, Kyoko; Shinkai, Shoji.

In: Immunity and Ageing, Vol. 5, 13, 24.10.2008.

Research output: Contribution to journalArticle

@article{e3c5e9e5fc46405e91f33ab790cd0602,
title = "The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly",
abstract = "Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.",
author = "Kishiko Ogawa and Katsuhiko Suzuki and Okutsu Mitsuharu and Kyoko Yamazaki and Shoji Shinkai",
year = "2008",
month = "10",
day = "24",
doi = "10.1186/1742-4933-5-13",
language = "English",
volume = "5",
journal = "Immunity and Ageing",
issn = "1742-4933",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly

AU - Ogawa, Kishiko

AU - Suzuki, Katsuhiko

AU - Mitsuharu, Okutsu

AU - Yamazaki, Kyoko

AU - Shinkai, Shoji

PY - 2008/10/24

Y1 - 2008/10/24

N2 - Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.

AB - Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.

UR - http://www.scopus.com/inward/record.url?scp=56349156642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56349156642&partnerID=8YFLogxK

U2 - 10.1186/1742-4933-5-13

DO - 10.1186/1742-4933-5-13

M3 - Article

VL - 5

JO - Immunity and Ageing

JF - Immunity and Ageing

SN - 1742-4933

M1 - 13

ER -