The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit

Vladimir V. Botchkarev, Valentina Rossio, Satoshi Yoshida

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polo-like kinases are important regulators of multiple mitotic events; however, how Polo-like kinases are spatially and temporally regulated to perform their many tasks is not well understood. Here, we examined the subcellular localization of the budding yeast Polo-like kinase Cdc5 using a functional Cdc5-GFP protein expressed from the endogenous locus. In addition to the well-described localization of Cdc5 at the spindle pole bodies (SPBs) and the bud neck, we found that Cdc5-GFP accumulates in the nucleus in early mitosis but is released to the cytoplasm in late mitosis in a manner dependent on the Cdc14 phosphatase. This Cdc5 release from the nucleus is important for mitotic exit because artificial sequestration of Cdc5 in the nucleus by addition of a strong nuclear localization signal (NLS) resulted in mitotic exit defects. We identified a key cytoplasmic target of Cdc5 as Bfa1, an inhibitor of mitotic exit. Our study revealed a novel layer of Cdc5 regulation and suggests the existence of a possible coordination between Cdc5 and Cdc14 activity.

Original languageEnglish
Pages (from-to)3260-3270
Number of pages11
JournalCell Cycle
Volume13
Issue number20
DOIs
Publication statusPublished - 2014 Oct 15
Externally publishedYes

Fingerprint

Anaphase
Saccharomycetales
Phosphotransferases
Mitosis
Spindle Pole Bodies
Nuclear Localization Signals
Phosphoric Monoester Hydrolases
Cytoplasm
Proteins

Keywords

  • Bfa1
  • Cdc14
  • Cdc5
  • FEAR
  • MEN
  • Mitosis
  • Mitotic exit
  • Polo-like kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit. / Botchkarev, Vladimir V.; Rossio, Valentina; Yoshida, Satoshi.

In: Cell Cycle, Vol. 13, No. 20, 15.10.2014, p. 3260-3270.

Research output: Contribution to journalArticle

Botchkarev, Vladimir V. ; Rossio, Valentina ; Yoshida, Satoshi. / The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit. In: Cell Cycle. 2014 ; Vol. 13, No. 20. pp. 3260-3270.
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