The catalytic RNA of RNase P from Escherichia coli cleaves Drosophila 2S ribosomal RNA in vitro

A new type of naturally occurring substrate for the ribozyme

Yoshiaki Hori, Terumichi Tanaka, Yo Kikuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have found that the catalytic RNA of RNase P of Escherichia coli (M1 RNA) can cleave 2S ribosomal RNA (2S rRNA) of Drosophila melanogaster at specific positions in vitro. The cleavage mainly occurred at two sites between nucleotides 11 and 12, and between 16 and 17 of 2S rRNA. Kinetic analyses of the reaction revealed that a dimer caused by intermolecular interaction of 2S rRNA may be the substrate for the cleavage between 11 and 12, while a simple monomer is the substrate for the cleavage between 16 and 17. Substrate recognition by M1 RNA is also discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalFEBS Letters
Volume472
Issue number2-3
DOIs
Publication statusPublished - 2000 Apr 28
Externally publishedYes

Fingerprint

Ribonuclease P
Catalytic RNA
Ribosomal RNA
Escherichia coli
Drosophila
Substrates
Drosophila melanogaster
Nucleotides
Dimers
Monomers
Kinetics
In Vitro Techniques

Keywords

  • Drosophila
  • Hyperprocessing
  • M1 RNA
  • Ribozyme
  • RNase P
  • rRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The catalytic RNA of RNase P from Escherichia coli cleaves Drosophila 2S ribosomal RNA in vitro : A new type of naturally occurring substrate for the ribozyme. / Hori, Yoshiaki; Tanaka, Terumichi; Kikuchi, Yo.

In: FEBS Letters, Vol. 472, No. 2-3, 28.04.2000, p. 187-190.

Research output: Contribution to journalArticle

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