The cholinergic system in the olfactory center of the terrestrial slug Limax

Ryota Matsuo, Suguru Kobayashi, Kyoko Wakiya, Miki Yamagishi, Masayuki Fukuoka, Etsuro Ito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acetylcholine plays various important roles in the central nervous system of invertebrates as well as vertebrates. In the olfactory center of the terrestrial slug Limax, the local field potential (LFP) oscillates, and the change in its oscillatory frequency is thought to correlate with the detection of odor that potentially changes an ongoing behavior of the animal. Acetylcholine is known to upregulate the frequency of the LFP oscillation, and is one of the candidates for the neurotransmitters that are involved in such higher cognitive functions. However, there have been no histological data on the cholinergic system in gastropods, nor are there data on the receptors that are responsible for the upregulation of the oscillatory frequency of LFP due to the lack of analytical tools (such as antibodies or cDNA sequence information on cholinergic system-related genes). Here we cloned the cDNAs of choline acetyltransferase (ChAT), acetylcholinesterase, vesicular acetylcholine transporter, and several nicotinic acetylcholine receptors (nAChRs), and investigated their localization in the brain of Limax. We also generated a polyclonal antibody against ChAT to examine its localization, and investigated pharmacologically the involvement of nAChRs in the LFP oscillation. Our data showed: 1) dense distribution of the neurons expressing mRNAs of ChAT and vesicular acetylcholine transporter in the olfactory center; 2) spatially unique expression patterns of different nAChRs in the olfactory center; 3) involvement of nAChRs in the upregulation of the oscillation; 4) localization of ChAT protein in nerve fibers and/or terminals; and 5) the presence of cholinergic nerves in the tentacles.

Original languageEnglish
Pages (from-to)2951-2966
Number of pages16
JournalJournal of Comparative Neurology
Volume522
Issue number13
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

Fingerprint

Gastropoda
Choline O-Acetyltransferase
Nicotinic Receptors
Cholinergic Agents
Vesicular Acetylcholine Transport Proteins
Up-Regulation
Acetylcholine
Complementary DNA
Animal Behavior
Antibodies
Invertebrates
Acetylcholinesterase
Nerve Fibers
Cognition
Neurotransmitter Agents
Vertebrates
Central Nervous System
Neurons
Messenger RNA
Brain

Keywords

  • Field potential oscillation
  • Nicotinic acetylcholine receptor
  • Olfaction
  • Procerebrum
  • Tentacle

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

The cholinergic system in the olfactory center of the terrestrial slug Limax. / Matsuo, Ryota; Kobayashi, Suguru; Wakiya, Kyoko; Yamagishi, Miki; Fukuoka, Masayuki; Ito, Etsuro.

In: Journal of Comparative Neurology, Vol. 522, No. 13, 01.09.2014, p. 2951-2966.

Research output: Contribution to journalArticle

Matsuo, R, Kobayashi, S, Wakiya, K, Yamagishi, M, Fukuoka, M & Ito, E 2014, 'The cholinergic system in the olfactory center of the terrestrial slug Limax', Journal of Comparative Neurology, vol. 522, no. 13, pp. 2951-2966. https://doi.org/10.1002/cne.23559
Matsuo, Ryota ; Kobayashi, Suguru ; Wakiya, Kyoko ; Yamagishi, Miki ; Fukuoka, Masayuki ; Ito, Etsuro. / The cholinergic system in the olfactory center of the terrestrial slug Limax. In: Journal of Comparative Neurology. 2014 ; Vol. 522, No. 13. pp. 2951-2966.
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