TY - JOUR
T1 - The Circulating Level of Klotho Is Not Dependent upon Physical Fitness and Age-Associated Methylation Increases at the Promoter Region of the Klotho Gene
AU - Aczel, Dora
AU - Torma, Ferenc
AU - Jokai, Matyas
AU - McGreevy, Kristen
AU - Boros, Anita
AU - Seki, Yasuhiro
AU - Boldogh, Istvan
AU - Horvath, Steve
AU - Radak, Zsolt
N1 - Funding Information:
ZR acknowledges support from the National Excellence Program (126823) and the Scientific Excellence Program, TKP2020-NKA-17, and TKP2021-EGA-37, at the Hungarian University of Sport Science, Ministry for Innovation and Technology, Hungary.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/2
Y1 - 2023/2
N2 - (1) Background: Higher levels of physical fitness are believed to increase the physiological quality of life and impact the aging process with a wide range of adaptive mechanisms, including the regulation of the expression of the age-associated klotho (KL) gene and protein levels. (2) Methods: Here, we tested the relationship between the DNA methylation-based epigenetic biomarkers PhenoAge and GrimAge and methylation of the promoter region of the KL gene, the circulating level of KL, and the stage of physical fitness and grip force in two groups of volunteer subjects, trained (TRND) and sedentary (SED), aged between 37 and 85 years old. (3) Results: The circulating KL level is negatively associated with chronological age in the TRND group (r = −0.19; p = 0.0295) but not in the SED group (r = −0.065; p = 0.5925). The age-associated decrease in circulating KL is partly due to the increased methylation of the KL gene. In addition, higher plasma KL is significantly related to epigenetic age-deceleration in the TRND group, assessed by the biomarker of PhenoAge (r = −0.21; p = 0.0192). (4) Conclusions: The level of physical fitness, on the other hand, does not relate to circulating KL levels, nor to the rate of the methylation of the promoter region of the KL gene, only in males.
AB - (1) Background: Higher levels of physical fitness are believed to increase the physiological quality of life and impact the aging process with a wide range of adaptive mechanisms, including the regulation of the expression of the age-associated klotho (KL) gene and protein levels. (2) Methods: Here, we tested the relationship between the DNA methylation-based epigenetic biomarkers PhenoAge and GrimAge and methylation of the promoter region of the KL gene, the circulating level of KL, and the stage of physical fitness and grip force in two groups of volunteer subjects, trained (TRND) and sedentary (SED), aged between 37 and 85 years old. (3) Results: The circulating KL level is negatively associated with chronological age in the TRND group (r = −0.19; p = 0.0295) but not in the SED group (r = −0.065; p = 0.5925). The age-associated decrease in circulating KL is partly due to the increased methylation of the KL gene. In addition, higher plasma KL is significantly related to epigenetic age-deceleration in the TRND group, assessed by the biomarker of PhenoAge (r = −0.21; p = 0.0192). (4) Conclusions: The level of physical fitness, on the other hand, does not relate to circulating KL levels, nor to the rate of the methylation of the promoter region of the KL gene, only in males.
KW - aging
KW - epigenetic clock
KW - klotho
KW - methylation
KW - physical fitness
UR - http://www.scopus.com/inward/record.url?scp=85148873473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85148873473&partnerID=8YFLogxK
U2 - 10.3390/genes14020525
DO - 10.3390/genes14020525
M3 - Article
C2 - 36833453
AN - SCOPUS:85148873473
SN - 2073-4425
VL - 14
JO - Genes
JF - Genes
IS - 2
M1 - 525
ER -