The complete genomic sequence of Niocardia farcinica IFM 10152

Jun Ishikawa, Atsushi Yamashita, Yuzuru Mikami, Yasutaka Hoshino, Haruyo Kurita, Kunimoto Hotta, Tadayoshi Shiba, Masahira Hattori

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNFl) and 87,093 (pNF2) bp with average G+C contents of 67.2% and 68.4%, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.

Original languageEnglish
Pages (from-to)14925-14930
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number41
DOIs
Publication statusPublished - 2004 Oct 12
Externally publishedYes

Fingerprint

Base Composition
Secondary Metabolism
MDR Genes
Nocardia
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 6
Gene Duplication
Virulence
Proteins
Plasmids
Soil
Genome
Bacteria

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The complete genomic sequence of Niocardia farcinica IFM 10152. / Ishikawa, Jun; Yamashita, Atsushi; Mikami, Yuzuru; Hoshino, Yasutaka; Kurita, Haruyo; Hotta, Kunimoto; Shiba, Tadayoshi; Hattori, Masahira.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 41, 12.10.2004, p. 14925-14930.

Research output: Contribution to journalArticle

Ishikawa, Jun ; Yamashita, Atsushi ; Mikami, Yuzuru ; Hoshino, Yasutaka ; Kurita, Haruyo ; Hotta, Kunimoto ; Shiba, Tadayoshi ; Hattori, Masahira. / The complete genomic sequence of Niocardia farcinica IFM 10152. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 41. pp. 14925-14930.
@article{e8e453a1c04c4bbba54c2af56d91dd4e,
title = "The complete genomic sequence of Niocardia farcinica IFM 10152",
abstract = "We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8{\%} and two plasmids of 184,027 (pNFl) and 87,093 (pNF2) bp with average G+C contents of 67.2{\%} and 68.4{\%}, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.",
author = "Jun Ishikawa and Atsushi Yamashita and Yuzuru Mikami and Yasutaka Hoshino and Haruyo Kurita and Kunimoto Hotta and Tadayoshi Shiba and Masahira Hattori",
year = "2004",
month = "10",
day = "12",
doi = "10.1073/pnas.0406410101",
language = "English",
volume = "101",
pages = "14925--14930",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "41",

}

TY - JOUR

T1 - The complete genomic sequence of Niocardia farcinica IFM 10152

AU - Ishikawa, Jun

AU - Yamashita, Atsushi

AU - Mikami, Yuzuru

AU - Hoshino, Yasutaka

AU - Kurita, Haruyo

AU - Hotta, Kunimoto

AU - Shiba, Tadayoshi

AU - Hattori, Masahira

PY - 2004/10/12

Y1 - 2004/10/12

N2 - We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNFl) and 87,093 (pNF2) bp with average G+C contents of 67.2% and 68.4%, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.

AB - We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNFl) and 87,093 (pNF2) bp with average G+C contents of 67.2% and 68.4%, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.

UR - http://www.scopus.com/inward/record.url?scp=6944252215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6944252215&partnerID=8YFLogxK

U2 - 10.1073/pnas.0406410101

DO - 10.1073/pnas.0406410101

M3 - Article

VL - 101

SP - 14925

EP - 14930

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 41

ER -