The curved DNA structure in the 5′-upstream region of the light-responsive genes

Its universality, binding factor and function for cyanobacterial psbA transcription

Munehiko Asayama, Hideki Kato, Junko Shibato, Makoto Shirai, Takashi Ohyama

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A unique DNA curvature, the CIT, has been found in the 5′-upstream region of the psbA2 gene, which exhibits basal, light-responsive and circadian rhythmic transcription, in a unicellular photosynthetic cyanobacterium, Microcystis aeruginosa K-81. In this study, we report the universality of curvatures found in 5′-upstream regions in the psbA family and the function of the curvature in gene expression. Intrinsic curvatures were identified within 1000 bp upstream from the psbA genes in another cyanobacterium, a red alga and in plants (monocot and dicot). Mutagenized curvatures were constructed and confirmed to have disrupted architecture by gel electrophoresis and atomic force microscopy. Relatively small amounts but light-responsive transcripts of psbA2 were observed in cyanobacterial transformants harboring the mutagenized curvature under light/dark and light/high-light conditions. This shows that the curvature is important for basal transcription. In vitro primer extension and DNA mobility shift assay revealed that factors which might bind to the region upstream from the bending center contribute to the effective basal transcription of psbA2.

Original languageEnglish
Pages (from-to)4658-4666
Number of pages9
JournalNucleic Acids Research
Volume30
Issue number21
Publication statusPublished - 2002 Nov 1
Externally publishedYes

Fingerprint

Light
DNA
Genes
Cyanobacteria
Microcystis
Rhodophyta
DNA Primers
Atomic Force Microscopy
Electrophoretic Mobility Shift Assay
Electrophoresis
Gels
Gene Expression

ASJC Scopus subject areas

  • Genetics

Cite this

The curved DNA structure in the 5′-upstream region of the light-responsive genes : Its universality, binding factor and function for cyanobacterial psbA transcription. / Asayama, Munehiko; Kato, Hideki; Shibato, Junko; Shirai, Makoto; Ohyama, Takashi.

In: Nucleic Acids Research, Vol. 30, No. 21, 01.11.2002, p. 4658-4666.

Research output: Contribution to journalArticle

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