The Dmrt1 expression in sex-reversed gonads of amphibians

Kazuyuki Shibata, Minoru Takase, Masahisa Nakamura

    Research output: Contribution to journalArticle

    98 Citations (Scopus)

    Abstract

    Gonadal differentiation in amphibians is sensitive to steroids. The phenotypic sex can be changed by hormonal treatments, but the molecular mechanism for gonadal differentiation is not known. Up to date, many genes involved in gonadal differentiation have been isolated in vertebrates. Dmrt1, a gene that contains the DM-domain (Doublesex/Mab-3 DNA-binding motif), is considered to be one of the essential genes involved in the testicular differentiation cascade in mammals, birds, reptiles, and fish. However, this gene has not been isolated in amphibians yet. To elucidate its role(s) for gonadal differentiation in vertebrates, a molecular cloning of Dmrt1 in amphibians is urgent. In this study, we have successfully isolated a Dmrt1 homolog from the frog Rana rugosa testis cDNA library and examined its expression during gonadal differentiation and in sex-reversed gonads. The Dmrt1 mRNA was exclusively detected in testis among adult tissues by the RT-PCR analysis. The Dmrt1 was first expressed in the differentiating testis at stage XXV in which spermatogonia are only germ cells, and became stronger at later stages. Moreover, the Dmrt1 transcript was not detected during ovarian differentiation. However, this gene was clearly expressed in XX sex-reversed gonads caused by injection of testosterone into all-female tadpoles that have well-differentiated ovaries. Taken together, the results suggest that Dmrt1 is closely implicated in testicular, but not ovarian differentiation in amphibians.

    Original languageEnglish
    Pages (from-to)232-241
    Number of pages10
    JournalGeneral and Comparative Endocrinology
    Volume127
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Gonads
    Amphibians
    amphibians
    gonads
    Testis
    testes
    gender
    Genes
    Vertebrates
    genes
    vertebrates
    Ranidae
    Sex Differentiation
    Spermatogonia
    Nucleotide Motifs
    Reptiles
    DNA-binding domains
    spermatogonia
    Essential Genes
    Molecular Cloning

    Keywords

    • Amphibians
    • Dmrt1
    • Sex-reversal
    • Testis
    • Testosterone

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    The Dmrt1 expression in sex-reversed gonads of amphibians. / Shibata, Kazuyuki; Takase, Minoru; Nakamura, Masahisa.

    In: General and Comparative Endocrinology, Vol. 127, No. 3, 2002, p. 232-241.

    Research output: Contribution to journalArticle

    Shibata, Kazuyuki ; Takase, Minoru ; Nakamura, Masahisa. / The Dmrt1 expression in sex-reversed gonads of amphibians. In: General and Comparative Endocrinology. 2002 ; Vol. 127, No. 3. pp. 232-241.
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