The DnaA homolog of the hyperthermophilic eubacterium Thermotoga maritima an open complex with a minimal 149-bp origin region in an ATP-dependent manner

Shogo Ozaki, Kazuyuki Fujimitsu, Hitoshi Kurumizaka, Tsutomu Katayama

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    In Escherichia coli, ATP-DnaA, but not ADP-DnaA, forms an initiation complex that undergoes site-specific duplex DNA unwinding, open complex formation. However, it remains unclear how highly the ATP-dependent activation of the initiation factor is conserved in evolution. The hyperthermophile Thermotoga maritima is one of the most ancient eubacteria in evolution. Here, we show that the DnaA homolog (tmaDnaA) of this bacterium forms open complexes with the predicted origin region (tma-oriC) in vitro. Tma DnaA has a strong and specific affinity for ATP/ADP as well as for 12-mer repeating sequences within the tma-oriC. Unlike ADPtma DnaA, ATPtma DnaA ishighly cooperative in DNA binding and forms open complexes in a manner that depends on temperature and the superhelical tension of the tma-oriC-bearing plasmid. The minimal tma-oriC required for unwinding is a 149-bp region containing five repeats of the 12-mer sequence and two AT-rich 9-mer repeats. Tma DnaA-binding to the 12-mer motif provokes DNA bending. The 9-mer region is the duplex-unwinding site. The tma DnaA-binding and unwinding motifs of tma-oriC share sequence homology with corresponding archaeal and eukaryotic sequences. These findings suggest that the ATP-dependent molecular switch of the initiator and the mechanisms in the replication initiation complex are highly conserved in eubacterial evolution.

    Original languageEnglish
    Pages (from-to)425-438
    Number of pages14
    JournalGenes to Cells
    Volume11
    Issue number4
    DOIs
    Publication statusPublished - 2006 Apr

    Fingerprint

    Thermotoga maritima
    Eubacterium
    Adenosine Triphosphate
    Adenosine Diphosphate
    AT Rich Sequence
    Bacteria
    Peptide Initiation Factors
    Nucleotide Motifs
    DNA
    Sequence Homology
    Plasmids
    Escherichia coli
    Temperature

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    Cite this

    The DnaA homolog of the hyperthermophilic eubacterium Thermotoga maritima an open complex with a minimal 149-bp origin region in an ATP-dependent manner. / Ozaki, Shogo; Fujimitsu, Kazuyuki; Kurumizaka, Hitoshi; Katayama, Tsutomu.

    In: Genes to Cells, Vol. 11, No. 4, 04.2006, p. 425-438.

    Research output: Contribution to journalArticle

    Ozaki, Shogo ; Fujimitsu, Kazuyuki ; Kurumizaka, Hitoshi ; Katayama, Tsutomu. / The DnaA homolog of the hyperthermophilic eubacterium Thermotoga maritima an open complex with a minimal 149-bp origin region in an ATP-dependent manner. In: Genes to Cells. 2006 ; Vol. 11, No. 4. pp. 425-438.
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