The effect of coenzyme Q10 included by γ-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy

Tatsuro Nishida, Tomohiro Kaino, Ryo Ikarashi, Daisuke Nakata, Keiji Terao, Masahiro Ando, Hiro O. Hamaguchi, Makoto Kawamukai, Tatsuyuki Yamamoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The inclusion complex of coenzyme Q10 (CoQ10) by γ-cyclodextrin (γ-CD), CoQ10-CD complex, was recently developed. The addition of the CoQ10-CD complex recovered the growth of a fission yeast mutant strain, δdps1, which otherwise cannot grow well due to the lack of coenzyme Q producing ability. However, the oxygen consumption rate of this strain was not restored by the addition of the CoQ10-CD complex. The addition of two other anti-oxidative reagents, glutathione and ascorbic acid, also recovered the growth of the δdps1 strain as well. These results indicated that the recovery of the growth of δdps1 was brought about by the anti-oxidative property of CoQ10. The intensity of Raman spectra of δdps1 at 1602 cm -1, which is prominently observed for the wild type of the fission yeast, was compared between before and after addition of the CoQ10-CD complex. The signal was very weakly observed for δdps1 and did not increase in intensity by the addition of the CoQ10-CD complex. These results suggested the recovery of the growth of δdps1 was brought about not by the restoration of respiration function of δdps1 but by the anti-oxidative property of CoQ10 to result in the decrease in the oxidative stress.

Original languageEnglish
Pages (from-to)375-381
Number of pages7
JournalJournal of Molecular Structure
Volume1048
DOIs
Publication statusPublished - 2013

Fingerprint

coenzyme Q10
Cyclodextrins
Yeast
Raman spectroscopy
Microscopes
Recovery
Ubiquinone
Oxidative stress

Keywords

  • Coenzyme Q10
  • Cyclodextrin
  • Fission yeast
  • Growth
  • Metabolic activity
  • Raman spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Analytical Chemistry
  • Inorganic Chemistry
  • Organic Chemistry

Cite this

The effect of coenzyme Q10 included by γ-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy. / Nishida, Tatsuro; Kaino, Tomohiro; Ikarashi, Ryo; Nakata, Daisuke; Terao, Keiji; Ando, Masahiro; Hamaguchi, Hiro O.; Kawamukai, Makoto; Yamamoto, Tatsuyuki.

In: Journal of Molecular Structure, Vol. 1048, 2013, p. 375-381.

Research output: Contribution to journalArticle

Nishida, Tatsuro ; Kaino, Tomohiro ; Ikarashi, Ryo ; Nakata, Daisuke ; Terao, Keiji ; Ando, Masahiro ; Hamaguchi, Hiro O. ; Kawamukai, Makoto ; Yamamoto, Tatsuyuki. / The effect of coenzyme Q10 included by γ-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy. In: Journal of Molecular Structure. 2013 ; Vol. 1048. pp. 375-381.
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