The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle

Evidence for beneficial outcomes

Zsolt Radak, Takao Kaneko, Shoichi Tahara, Hideko Nakamoto, Hideki Ohno, Mária Sasvári, Csaba Nyakas, Sataro Goto

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Moderate daily exercise is known to be beneficial to health, reducing risks of a number of age-related disorders. Molecular mechanisms that bring about these effects are not clear. In contrast, it has been claimed that some types of prolonged physical exertion are detrimental to health because active oxygen species are generated excessively by enhanced oxygen consumption. Using two age groups of rats, young (4 week) and middle aged (14 months), we investigated the effects of long-term swimming training on the oxidative status of phospholipids, proteins, and DNA. The concentration of thiobarbituric acid reactive substances and 4-hydroxynonenal protein adducts did not differ in the gastrocnemius muscle between exercised and nonexercised animals in the two age groups. The extent of carbonylation in a protein of molecular-weight around 29 KDa and the amount of 8-hydroxydeoxyguanosine in nuclear DNA were smaller (p < .05) in the exercised rats than in the sedentary animals. Activities of DT-diaphorase (CI: 29.3 ± 1.9; C2: 36.1 ± 2.6; El: 27.2 ± 1.3; C2: 33.4 ± 2.9 nmol/mg protein) and proteasome, a major proteolytic enzyme for oxidatively modified proteins were significantly higher in the exercised animals of both age groups (p < .05). The adaptive response against oxidative stress induced by moderate endurance exercise constitutes a beneficial effect of exercise.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalFree Radical Biology and Medicine
Volume27
Issue number1-2
DOIs
Publication statusPublished - 1999 Jul 1
Externally publishedYes

Fingerprint

Muscle
Rats
Skeletal Muscle
Exercise
Lipids
DNA
Animals
Age Groups
Proteins
Health
NAD(P)H Dehydrogenase (Quinone)
Physical Exertion
Carbonylation
Oxidative stress
Thiobarbituric Acid Reactive Substances
Proteasome Endopeptidase Complex
Oxygen Consumption
Reactive Oxygen Species
Phospholipids
Durability

Keywords

  • DT-diaphorase
  • Exercise
  • Free radicals
  • Oxidative damage
  • Proteasome complex
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle : Evidence for beneficial outcomes. / Radak, Zsolt; Kaneko, Takao; Tahara, Shoichi; Nakamoto, Hideko; Ohno, Hideki; Sasvári, Mária; Nyakas, Csaba; Goto, Sataro.

In: Free Radical Biology and Medicine, Vol. 27, No. 1-2, 01.07.1999, p. 69-74.

Research output: Contribution to journalArticle

Radak, Zsolt ; Kaneko, Takao ; Tahara, Shoichi ; Nakamoto, Hideko ; Ohno, Hideki ; Sasvári, Mária ; Nyakas, Csaba ; Goto, Sataro. / The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle : Evidence for beneficial outcomes. In: Free Radical Biology and Medicine. 1999 ; Vol. 27, No. 1-2. pp. 69-74.
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