The effect of night shift work on the expression of clock genes in beard hair follicle cells

Miwa Hattammaru, Yu Tahara, Tomoko Kikuchi, Kiyotaka Okajima, Koichi Konishi, Shun Nakajima, Kyoko Sato, Kuniaki Otsuka, Hiroshi Sakura, Shigenobu Shibata, Takashi Nakaoka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: Shift work encompasses a broad range of work time arrangements. However, how shift work affects the circadian expression of clock genes remains to be explored. The objective of this study was to evaluate the pattern of clock gene expression in shift workers in the field. Methods: We examined clock gene expression in Japanese men who work: (1) one night shift followed by a day off (caregivers: nurses and doctors; the one-night group); (2) three or more consecutive night shifts (factory workers; the consecutive-night group); or (3) daytime only (the daytime group), using beard follicle samples. The expression of Period3, Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), and Nuclear Receptor Subfamily 1 Group D Member 2 (Nr1d2) was examined by real-time polymerase chain reaction. Results: Period3 expression in the daytime and one-night groups together with Nr1d2 expression in the one-night group fitted a 24-h-period cosine curve better than in the consecutive-night group (p = 0.004, 0.012, and 0.001, respectively). The level of overall Period3 gene expression, calibrated with that of 18S-rRNA, was decreased in the consecutive-night group compared with that in the daytime group (p = 0.006). The patterns of Period3 and Nr1d2 expression in the daytime and one-night groups were more coherent than those in the consecutive-night group. Conclusions: These results suggest that night shift work affects the rhythms and levels of circadian Period3 and Nr1d2 expression dependent on the shift schedule or type of the shift; however, there is substantial variation between individuals.

Original languageEnglish
JournalSleep Medicine
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Hair Follicle
Gene Expression
Nuclear Receptor Subfamily 1, Group D, Member 1
Circadian Clocks
Cytoplasmic and Nuclear Receptors
Circadian Rhythm
Health Personnel
Caregivers
Real-Time Polymerase Chain Reaction
Appointments and Schedules

Keywords

  • Circadian rhythm
  • Clock gene
  • Period3
  • Real-time polymerase chain reaction
  • Shift work

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The effect of night shift work on the expression of clock genes in beard hair follicle cells. / Hattammaru, Miwa; Tahara, Yu; Kikuchi, Tomoko; Okajima, Kiyotaka; Konishi, Koichi; Nakajima, Shun; Sato, Kyoko; Otsuka, Kuniaki; Sakura, Hiroshi; Shibata, Shigenobu; Nakaoka, Takashi.

In: Sleep Medicine, 01.01.2019.

Research output: Contribution to journalArticle

Hattammaru, M, Tahara, Y, Kikuchi, T, Okajima, K, Konishi, K, Nakajima, S, Sato, K, Otsuka, K, Sakura, H, Shibata, S & Nakaoka, T 2019, 'The effect of night shift work on the expression of clock genes in beard hair follicle cells', Sleep Medicine. https://doi.org/10.1016/j.sleep.2019.01.005
Hattammaru, Miwa ; Tahara, Yu ; Kikuchi, Tomoko ; Okajima, Kiyotaka ; Konishi, Koichi ; Nakajima, Shun ; Sato, Kyoko ; Otsuka, Kuniaki ; Sakura, Hiroshi ; Shibata, Shigenobu ; Nakaoka, Takashi. / The effect of night shift work on the expression of clock genes in beard hair follicle cells. In: Sleep Medicine. 2019.
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abstract = "Objective: Shift work encompasses a broad range of work time arrangements. However, how shift work affects the circadian expression of clock genes remains to be explored. The objective of this study was to evaluate the pattern of clock gene expression in shift workers in the field. Methods: We examined clock gene expression in Japanese men who work: (1) one night shift followed by a day off (caregivers: nurses and doctors; the one-night group); (2) three or more consecutive night shifts (factory workers; the consecutive-night group); or (3) daytime only (the daytime group), using beard follicle samples. The expression of Period3, Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), and Nuclear Receptor Subfamily 1 Group D Member 2 (Nr1d2) was examined by real-time polymerase chain reaction. Results: Period3 expression in the daytime and one-night groups together with Nr1d2 expression in the one-night group fitted a 24-h-period cosine curve better than in the consecutive-night group (p = 0.004, 0.012, and 0.001, respectively). The level of overall Period3 gene expression, calibrated with that of 18S-rRNA, was decreased in the consecutive-night group compared with that in the daytime group (p = 0.006). The patterns of Period3 and Nr1d2 expression in the daytime and one-night groups were more coherent than those in the consecutive-night group. Conclusions: These results suggest that night shift work affects the rhythms and levels of circadian Period3 and Nr1d2 expression dependent on the shift schedule or type of the shift; however, there is substantial variation between individuals.",
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AU - Okajima, Kiyotaka

AU - Konishi, Koichi

AU - Nakajima, Shun

AU - Sato, Kyoko

AU - Otsuka, Kuniaki

AU - Sakura, Hiroshi

AU - Shibata, Shigenobu

AU - Nakaoka, Takashi

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N2 - Objective: Shift work encompasses a broad range of work time arrangements. However, how shift work affects the circadian expression of clock genes remains to be explored. The objective of this study was to evaluate the pattern of clock gene expression in shift workers in the field. Methods: We examined clock gene expression in Japanese men who work: (1) one night shift followed by a day off (caregivers: nurses and doctors; the one-night group); (2) three or more consecutive night shifts (factory workers; the consecutive-night group); or (3) daytime only (the daytime group), using beard follicle samples. The expression of Period3, Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), and Nuclear Receptor Subfamily 1 Group D Member 2 (Nr1d2) was examined by real-time polymerase chain reaction. Results: Period3 expression in the daytime and one-night groups together with Nr1d2 expression in the one-night group fitted a 24-h-period cosine curve better than in the consecutive-night group (p = 0.004, 0.012, and 0.001, respectively). The level of overall Period3 gene expression, calibrated with that of 18S-rRNA, was decreased in the consecutive-night group compared with that in the daytime group (p = 0.006). The patterns of Period3 and Nr1d2 expression in the daytime and one-night groups were more coherent than those in the consecutive-night group. Conclusions: These results suggest that night shift work affects the rhythms and levels of circadian Period3 and Nr1d2 expression dependent on the shift schedule or type of the shift; however, there is substantial variation between individuals.

AB - Objective: Shift work encompasses a broad range of work time arrangements. However, how shift work affects the circadian expression of clock genes remains to be explored. The objective of this study was to evaluate the pattern of clock gene expression in shift workers in the field. Methods: We examined clock gene expression in Japanese men who work: (1) one night shift followed by a day off (caregivers: nurses and doctors; the one-night group); (2) three or more consecutive night shifts (factory workers; the consecutive-night group); or (3) daytime only (the daytime group), using beard follicle samples. The expression of Period3, Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), and Nuclear Receptor Subfamily 1 Group D Member 2 (Nr1d2) was examined by real-time polymerase chain reaction. Results: Period3 expression in the daytime and one-night groups together with Nr1d2 expression in the one-night group fitted a 24-h-period cosine curve better than in the consecutive-night group (p = 0.004, 0.012, and 0.001, respectively). The level of overall Period3 gene expression, calibrated with that of 18S-rRNA, was decreased in the consecutive-night group compared with that in the daytime group (p = 0.006). The patterns of Period3 and Nr1d2 expression in the daytime and one-night groups were more coherent than those in the consecutive-night group. Conclusions: These results suggest that night shift work affects the rhythms and levels of circadian Period3 and Nr1d2 expression dependent on the shift schedule or type of the shift; however, there is substantial variation between individuals.

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