The effect of surface charge property on Escherichia coli initial adhesion and subsequent biofilm formation

Akihiko Terada, Keisuke Okuyama, Megumi Nishikawa, Satoshi Tsuneda, Masaaki Hosomi

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Polyethylene (PE) sheets were modified by radiation-induced graft polymerization (RIGP) of an epoxy-group containing monomer glycidyl methacrylate (GMA). The epoxy group of GMA was opened by introducing sodium sulfite (SS) and diethylamine (DEA) as representatives of negatively and positively charged functional groups, respectively. These modified surfaces by RIGP, termed GMA, SS, and DEA sheets, were investigated to elucidate their effects on initial adhesion and subsequent biofilm formation of Escherichia coli. Initial adhesion test revealed that E. coli density and viability were governed by sheet surface electrostatic property: E. coli cell density on the DEA sheet was 23 times higher than that on the SS sheet after 8h incubation. The viability of E. coli cells dramatically decreased after contact with the DEA sheet, but remained high on the SS sheet. E. coli biofilm structure on the DEA sheet was dense, homogeneous, and uniform, with biomass higher than that of the GMA and SS sheets by factors of 14.0 and 37.5, respectively. On the contrary, biofilm structure on the SS sheet was sparse, heterogeneous, and mushroom-shaped. More than 40% of E. coli biofilm on the DEA sheet was retained under a high liquid shear force condition (5,000s -1), whereas 97% and 100% of biofilms on the GMA and SS sheets were sloughed, indicating that E. coli biofilm robustness depends on surface charge property of the substratum. This suggests that substratum surface fabrication by RIGP may enhance or suppress biofilm formation, a finding with potentially important practical implications.

Original languageEnglish
Pages (from-to)1745-1754
Number of pages10
JournalBiotechnology and bioengineering
Volume109
Issue number7
DOIs
Publication statusPublished - 2012 Jul

Keywords

  • Biofilm
  • Electrostatic interaction
  • Escherichia coli
  • Initial bacterial adhesion
  • Radiation-induced graft polymerization
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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