The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo

Iliyana Ilieva, Kazuhiro Ohgami, Kenji Shiratori, Yoshikazu Koyama, Kazuhiko Yoshida, Satoru Kase, Hirokuni Kitamei, Yuko Takemoto, Kazunaga Yazawa, Shigeaki Ohno

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Purpose. Ginkgo biloba extract (GBE) contains many different flavone glycosides and terpenoides. Several previous studies have demonstrated that GBE exhibits a wide variety of biological activities, including an antioxidant action, on which we focused our attention. The aim of the present study was to investigate the efficacy of GBE on endotoxin induced uveitis in rats. The anti-inflammatory potency of GBE in vivo was compared with that of prednisolone. In addition, we also investigated nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and the expression of iNOS in a mouse macrophage cell line (RAW 264·7) treated with GBE in vitro to clarify the anti-inflammatory effect. Methods. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, either 1, 10 or 100 μg of GBE were injected intravenously. 24hr later, the aqueous humor was collected from both eyes, and the number of infiltrating cells, protein concentration and NO level in the aqueous humor was determined. The RAW 264·7 cells were pretreated with various concentrations of GBE for 24hr and subsequently incubated with LPS for 24hr. Levels of NO, PGE2 and TNF-α were determined by enzyme-linked immunosorbent assay. The expression of iNOS protein was analyzed by Western blotting method. Results. GBE treatment in vivo decreased the concentrations of protein and NO in the aqueous humor of EIU rats. The anti-inflammatory effect of 1 mg GBE was as strong as that of same dose prednisolone. It also significantly reduced the concentration of PGE2, TNF-α and NO production in the medium of RAW 264·7 cells compared to that of the LPS group in vitro. The expression of iNOS protein in the 1000 μg ml -1 of GBE treated cells decreased significantly. Conclusion. The present results indicate GBE suppresses the inflammation of EIU by blocking the iNOS protein expression and its anti-inflammatory effect on eye is comparable with the effect of prednisolone used in similar doses.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalExperimental Eye Research
Volume79
Issue number2
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

Fingerprint

Ginkgo biloba
Lipopolysaccharides
Inflammation
Nitric Oxide
Aqueous Humor
Anti-Inflammatory Agents
Prednisolone
Dinoprostone
flavone
Tumor Necrosis Factor-alpha
Proteins
In Vitro Techniques
Uveitis
Glycosides
Cell Extracts
Endotoxins
Cell Count
Antioxidants
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • anti-inflammatory agent
  • ginkgo biloba extract
  • inducible nitricoxide synthase
  • nitric oxide
  • uveitis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Ilieva, I., Ohgami, K., Shiratori, K., Koyama, Y., Yoshida, K., Kase, S., ... Ohno, S. (2004). The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo. Experimental Eye Research, 79(2), 181-187. https://doi.org/10.1016/j.exer.2004.03.009

The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo. / Ilieva, Iliyana; Ohgami, Kazuhiro; Shiratori, Kenji; Koyama, Yoshikazu; Yoshida, Kazuhiko; Kase, Satoru; Kitamei, Hirokuni; Takemoto, Yuko; Yazawa, Kazunaga; Ohno, Shigeaki.

In: Experimental Eye Research, Vol. 79, No. 2, 08.2004, p. 181-187.

Research output: Contribution to journalArticle

Ilieva, I, Ohgami, K, Shiratori, K, Koyama, Y, Yoshida, K, Kase, S, Kitamei, H, Takemoto, Y, Yazawa, K & Ohno, S 2004, 'The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo', Experimental Eye Research, vol. 79, no. 2, pp. 181-187. https://doi.org/10.1016/j.exer.2004.03.009
Ilieva, Iliyana ; Ohgami, Kazuhiro ; Shiratori, Kenji ; Koyama, Yoshikazu ; Yoshida, Kazuhiko ; Kase, Satoru ; Kitamei, Hirokuni ; Takemoto, Yuko ; Yazawa, Kazunaga ; Ohno, Shigeaki. / The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo. In: Experimental Eye Research. 2004 ; Vol. 79, No. 2. pp. 181-187.
@article{f69bcd1824044f2584a6ba6dcd18ff0f,
title = "The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo",
abstract = "Purpose. Ginkgo biloba extract (GBE) contains many different flavone glycosides and terpenoides. Several previous studies have demonstrated that GBE exhibits a wide variety of biological activities, including an antioxidant action, on which we focused our attention. The aim of the present study was to investigate the efficacy of GBE on endotoxin induced uveitis in rats. The anti-inflammatory potency of GBE in vivo was compared with that of prednisolone. In addition, we also investigated nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and the expression of iNOS in a mouse macrophage cell line (RAW 264·7) treated with GBE in vitro to clarify the anti-inflammatory effect. Methods. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, either 1, 10 or 100 μg of GBE were injected intravenously. 24hr later, the aqueous humor was collected from both eyes, and the number of infiltrating cells, protein concentration and NO level in the aqueous humor was determined. The RAW 264·7 cells were pretreated with various concentrations of GBE for 24hr and subsequently incubated with LPS for 24hr. Levels of NO, PGE2 and TNF-α were determined by enzyme-linked immunosorbent assay. The expression of iNOS protein was analyzed by Western blotting method. Results. GBE treatment in vivo decreased the concentrations of protein and NO in the aqueous humor of EIU rats. The anti-inflammatory effect of 1 mg GBE was as strong as that of same dose prednisolone. It also significantly reduced the concentration of PGE2, TNF-α and NO production in the medium of RAW 264·7 cells compared to that of the LPS group in vitro. The expression of iNOS protein in the 1000 μg ml -1 of GBE treated cells decreased significantly. Conclusion. The present results indicate GBE suppresses the inflammation of EIU by blocking the iNOS protein expression and its anti-inflammatory effect on eye is comparable with the effect of prednisolone used in similar doses.",
keywords = "anti-inflammatory agent, ginkgo biloba extract, inducible nitricoxide synthase, nitric oxide, uveitis",
author = "Iliyana Ilieva and Kazuhiro Ohgami and Kenji Shiratori and Yoshikazu Koyama and Kazuhiko Yoshida and Satoru Kase and Hirokuni Kitamei and Yuko Takemoto and Kazunaga Yazawa and Shigeaki Ohno",
year = "2004",
month = "8",
doi = "10.1016/j.exer.2004.03.009",
language = "English",
volume = "79",
pages = "181--187",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo

AU - Ilieva, Iliyana

AU - Ohgami, Kazuhiro

AU - Shiratori, Kenji

AU - Koyama, Yoshikazu

AU - Yoshida, Kazuhiko

AU - Kase, Satoru

AU - Kitamei, Hirokuni

AU - Takemoto, Yuko

AU - Yazawa, Kazunaga

AU - Ohno, Shigeaki

PY - 2004/8

Y1 - 2004/8

N2 - Purpose. Ginkgo biloba extract (GBE) contains many different flavone glycosides and terpenoides. Several previous studies have demonstrated that GBE exhibits a wide variety of biological activities, including an antioxidant action, on which we focused our attention. The aim of the present study was to investigate the efficacy of GBE on endotoxin induced uveitis in rats. The anti-inflammatory potency of GBE in vivo was compared with that of prednisolone. In addition, we also investigated nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and the expression of iNOS in a mouse macrophage cell line (RAW 264·7) treated with GBE in vitro to clarify the anti-inflammatory effect. Methods. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, either 1, 10 or 100 μg of GBE were injected intravenously. 24hr later, the aqueous humor was collected from both eyes, and the number of infiltrating cells, protein concentration and NO level in the aqueous humor was determined. The RAW 264·7 cells were pretreated with various concentrations of GBE for 24hr and subsequently incubated with LPS for 24hr. Levels of NO, PGE2 and TNF-α were determined by enzyme-linked immunosorbent assay. The expression of iNOS protein was analyzed by Western blotting method. Results. GBE treatment in vivo decreased the concentrations of protein and NO in the aqueous humor of EIU rats. The anti-inflammatory effect of 1 mg GBE was as strong as that of same dose prednisolone. It also significantly reduced the concentration of PGE2, TNF-α and NO production in the medium of RAW 264·7 cells compared to that of the LPS group in vitro. The expression of iNOS protein in the 1000 μg ml -1 of GBE treated cells decreased significantly. Conclusion. The present results indicate GBE suppresses the inflammation of EIU by blocking the iNOS protein expression and its anti-inflammatory effect on eye is comparable with the effect of prednisolone used in similar doses.

AB - Purpose. Ginkgo biloba extract (GBE) contains many different flavone glycosides and terpenoides. Several previous studies have demonstrated that GBE exhibits a wide variety of biological activities, including an antioxidant action, on which we focused our attention. The aim of the present study was to investigate the efficacy of GBE on endotoxin induced uveitis in rats. The anti-inflammatory potency of GBE in vivo was compared with that of prednisolone. In addition, we also investigated nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and the expression of iNOS in a mouse macrophage cell line (RAW 264·7) treated with GBE in vitro to clarify the anti-inflammatory effect. Methods. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). Immediately after the LPS inoculation, either 1, 10 or 100 μg of GBE were injected intravenously. 24hr later, the aqueous humor was collected from both eyes, and the number of infiltrating cells, protein concentration and NO level in the aqueous humor was determined. The RAW 264·7 cells were pretreated with various concentrations of GBE for 24hr and subsequently incubated with LPS for 24hr. Levels of NO, PGE2 and TNF-α were determined by enzyme-linked immunosorbent assay. The expression of iNOS protein was analyzed by Western blotting method. Results. GBE treatment in vivo decreased the concentrations of protein and NO in the aqueous humor of EIU rats. The anti-inflammatory effect of 1 mg GBE was as strong as that of same dose prednisolone. It also significantly reduced the concentration of PGE2, TNF-α and NO production in the medium of RAW 264·7 cells compared to that of the LPS group in vitro. The expression of iNOS protein in the 1000 μg ml -1 of GBE treated cells decreased significantly. Conclusion. The present results indicate GBE suppresses the inflammation of EIU by blocking the iNOS protein expression and its anti-inflammatory effect on eye is comparable with the effect of prednisolone used in similar doses.

KW - anti-inflammatory agent

KW - ginkgo biloba extract

KW - inducible nitricoxide synthase

KW - nitric oxide

KW - uveitis

UR - http://www.scopus.com/inward/record.url?scp=4344696660&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344696660&partnerID=8YFLogxK

U2 - 10.1016/j.exer.2004.03.009

DO - 10.1016/j.exer.2004.03.009

M3 - Article

C2 - 15325565

AN - SCOPUS:4344696660

VL - 79

SP - 181

EP - 187

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 2

ER -