The effects of interferon- α on oxygen radical production by human neutrophils

Takuya Koie, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Qiang Liu, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study was done to determine if the production and metabolism of reactive oxygen species from human neutrophils were modulated by the treatment of interferon- α (IFN- α). Luminol-dependent Chemiluminescence(LmCL) responses were inhibited by a high concentration of IFN- α (more than 1 X 104IU/ml)when opsonized zymosan(OZ) and phorbol 12- myristate 13-acetate(PMA) were used as stimulants. However, these responses were increased by 1 X 103IU/ml of IFN- α with Ca2+-ionophore A23187 stimulation. Lucigenin-dependent Chemiluminescence(LgCL) responses were inhibited by all concentrations. These findings suggest the possibility that IFN- α inhibits activation of protein kinase C(PKC), whereas the resulting effect might be due to the inhibition of myeloperoxidese(MPO) degranulation. Preincubation of human neutrophils with IFN- α for 30,60 or 120 minutes and subsequent stimulation with OZ, PMA and Ca2+-ionophore A23187 caused an increase LgCL responses, while inhibiting LmCL responses. These findings suggest that preincubation of human neutrophils with a high concentration of IFN- α might enhance the NADPH-oxidase activity, although a relative increase of LgCL was due to the inhibition MPO degranulation.

Original languageEnglish
Pages (from-to)536-544
Number of pages9
JournalJapanese Journal of Hygiene
Volume53
Issue number3
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Interferons
Luminescence
Reactive Oxygen Species
Neutrophils
Luminol
Zymosan
Ionophores
Calcimycin
Acetates
NADPH Oxidase
Protein Kinase C
10,10'-dimethyl-9,9'-biacridinium
phorbol-12-myristate

Keywords

  • Interferon- α
  • Neutrophil
  • Reactive oxygen species

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Koie, T., Suzuki, K., Kudo, S., Yamada, M., Liu, Q., Nakaji, S., & Sugawara, K. (1998). The effects of interferon- α on oxygen radical production by human neutrophils. Japanese Journal of Hygiene, 53(3), 536-544.

The effects of interferon- α on oxygen radical production by human neutrophils. / Koie, Takuya; Suzuki, Katsuhiko; Kudo, Satoru; Yamada, Mutsuo; Liu, Qiang; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Japanese Journal of Hygiene, Vol. 53, No. 3, 1998, p. 536-544.

Research output: Contribution to journalArticle

Koie, T, Suzuki, K, Kudo, S, Yamada, M, Liu, Q, Nakaji, S & Sugawara, K 1998, 'The effects of interferon- α on oxygen radical production by human neutrophils', Japanese Journal of Hygiene, vol. 53, no. 3, pp. 536-544.
Koie, Takuya ; Suzuki, Katsuhiko ; Kudo, Satoru ; Yamada, Mutsuo ; Liu, Qiang ; Nakaji, Shigeyuki ; Sugawara, Kazuo. / The effects of interferon- α on oxygen radical production by human neutrophils. In: Japanese Journal of Hygiene. 1998 ; Vol. 53, No. 3. pp. 536-544.
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