The effects of TiO2 nanoparticles on cisplatin cytotoxicity in cancer cell lines

Basma Salama, El Said El-Sherbini, Gehad El-Sayed, Mohamed El-Adl, Koki Kanehira, Akiyoshi Taniguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

There have been many studies on improving the efficacy of cisplatin and on identifying safe compounds that can overcome multi-drug resistance (MDR) acquired by cancer cells. Our previous research showed that polyethylene glycol-modified titanium dioxide nanoparticles (TiO2 PEG NPs) affect cell membrane receptors, resulting in their aggregation, altered localization and downregulation. TiO2 PEG NPs may affect P-glycoprotein (P-gp), a membrane efflux channel involved in MDR. In this study, we investigated the effect of TiO2 PEG NPs on cisplatin cytotoxicity. We used HepG2 cells, which highly express P-gp and A431 cells, which show low expression of P-gp. The results showed that 10 µg/mL 100 nm TiO2 PEG NPs increased intracellular cisplatin levels and cytotoxicity in HepG2 cells but not in A431 cells. TiO2 PEG NPs treatment decreased the expression level of P-gp in HepG2 cells. Our findings indicate that TiO2 PEG NPs enhance cisplatin cytotoxicity by down regulating P-gp and that TiO2 PEG NPs are promising candidates for inhibiting P-gp and reversing drug resistance acquired by cancer cells.

Original languageEnglish
Article number605
JournalInternational journal of molecular sciences
Volume21
Issue number2
DOIs
Publication statusPublished - 2020 Jan 2
Externally publishedYes

Keywords

  • Cisplatin
  • Cytotoxicity
  • Drug resistance
  • P-glycoprotein
  • Titanium dioxide nanoparticles

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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