The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling

Masamitsu Sato; Satoko Shinozaki-Yabana; Akira Yamashita; Yoshinori Watanabe; Masayuki Yamamoto

doi:10.1016/S0014-5793(01)02567-4

The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling

Masamitsu Sato, Satoko Shinozaki-Yabana, Akira Yamashita, Yoshinori Watanabe, Masayuki Yamamoto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

Original language	English
Pages (from-to)	251-255
Number of pages	5
Journal	FEBS Letters
Volume	499
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)02567-4
Publication status	Published - 2001 Jun 22
Externally published	Yes

Keywords

Meiosis
Nuclear export
Nuclear import
Nucleocytoplasmic shuttling
RNA-binding protein
meiRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02567-4

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title = "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling",

abstract = "Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.",

keywords = "Meiosis, Nuclear export, Nuclear import, Nucleocytoplasmic shuttling, RNA-binding protein, meiRNA",

author = "Masamitsu Sato and Satoko Shinozaki-Yabana and Akira Yamashita and Yoshinori Watanabe and Masayuki Yamamoto",

note = "Funding Information: We thank M. Yoshida for supplying LMB, M. Yanagida for the crm1 strain, and N. Kudo for advice. This work was supported by a Grant-in-Aid for Specially Promoted Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Mitsubishi Foundation.",

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doi = "10.1016/S0014-5793(01)02567-4",

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volume = "499",

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AU - Sato, Masamitsu

AU - Shinozaki-Yabana, Satoko

AU - Yamashita, Akira

AU - Watanabe, Yoshinori

AU - Yamamoto, Masayuki

N1 - Funding Information: We thank M. Yoshida for supplying LMB, M. Yanagida for the crm1 strain, and N. Kudo for advice. This work was supported by a Grant-in-Aid for Specially Promoted Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Mitsubishi Foundation.

PY - 2001/6/22

Y1 - 2001/6/22

N2 - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

AB - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

KW - Meiosis

KW - Nuclear export

KW - Nuclear import

KW - Nucleocytoplasmic shuttling

KW - RNA-binding protein

KW - meiRNA

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VL - 499

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -

The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling

Abstract

Keywords

ASJC Scopus subject areas

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