Abstract
Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.
| Original language | English |
|---|---|
| Pages (from-to) | 251-255 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 499 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 Jun 22 |
| Externally published | Yes |
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Keywords
- Meiosis
- meiRNA
- Nuclear export
- Nuclear import
- Nucleocytoplasmic shuttling
- RNA-binding protein
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling. / Sato, Masamitsu; Shinozaki-Yabana, Satoko; Yamashita, Akira; Watanabe, Yoshinori; Yamamoto, Masayuki.
In: FEBS Letters, Vol. 499, No. 3, 22.06.2001, p. 251-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling
AU - Sato, Masamitsu
AU - Shinozaki-Yabana, Satoko
AU - Yamashita, Akira
AU - Watanabe, Yoshinori
AU - Yamamoto, Masayuki
PY - 2001/6/22
Y1 - 2001/6/22
N2 - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.
AB - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.
KW - Meiosis
KW - meiRNA
KW - Nuclear export
KW - Nuclear import
KW - Nucleocytoplasmic shuttling
KW - RNA-binding protein
UR - http://www.scopus.com/inward/record.url?scp=0035933599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035933599&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(01)02567-4
DO - 10.1016/S0014-5793(01)02567-4
M3 - Article
C2 - 11423126
AN - SCOPUS:0035933599
VL - 499
SP - 251
EP - 255
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -