The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling

Masamitsu Sato, Satoko Shinozaki-Yabana, Akira Yamashita, Yoshinori Watanabe, Masayuki Yamamoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalFEBS Letters
Volume499
Issue number3
DOIs
Publication statusPublished - 2001 Jun 22
Externally publishedYes

Fingerprint

Untranslated RNA
RNA-Binding Proteins
Schizosaccharomyces
Meiosis
Yeast
Cell Cycle
Cells
Switches
RNA
Molecules
Proteins

Keywords

  • Meiosis
  • meiRNA
  • Nuclear export
  • Nuclear import
  • Nucleocytoplasmic shuttling
  • RNA-binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sato, M., Shinozaki-Yabana, S., Yamashita, A., Watanabe, Y., & Yamamoto, M. (2001). The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling. FEBS Letters, 499(3), 251-255. https://doi.org/10.1016/S0014-5793(01)02567-4

The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling. / Sato, Masamitsu; Shinozaki-Yabana, Satoko; Yamashita, Akira; Watanabe, Yoshinori; Yamamoto, Masayuki.

In: FEBS Letters, Vol. 499, No. 3, 22.06.2001, p. 251-255.

Research output: Contribution to journalArticle

Sato, M, Shinozaki-Yabana, S, Yamashita, A, Watanabe, Y & Yamamoto, M 2001, 'The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling', FEBS Letters, vol. 499, no. 3, pp. 251-255. https://doi.org/10.1016/S0014-5793(01)02567-4
Sato, Masamitsu ; Shinozaki-Yabana, Satoko ; Yamashita, Akira ; Watanabe, Yoshinori ; Yamamoto, Masayuki. / The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling. In: FEBS Letters. 2001 ; Vol. 499, No. 3. pp. 251-255.
@article{ea3f43a0cbe3430284a926614fa724a4,
title = "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling",
abstract = "Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.",
keywords = "Meiosis, meiRNA, Nuclear export, Nuclear import, Nucleocytoplasmic shuttling, RNA-binding protein",
author = "Masamitsu Sato and Satoko Shinozaki-Yabana and Akira Yamashita and Yoshinori Watanabe and Masayuki Yamamoto",
year = "2001",
month = "6",
day = "22",
doi = "10.1016/S0014-5793(01)02567-4",
language = "English",
volume = "499",
pages = "251--255",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling

AU - Sato, Masamitsu

AU - Shinozaki-Yabana, Satoko

AU - Yamashita, Akira

AU - Watanabe, Yoshinori

AU - Yamamoto, Masayuki

PY - 2001/6/22

Y1 - 2001/6/22

N2 - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

AB - Schizosaccharomyces pombe Mei2p is an RNA-binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non-coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.

KW - Meiosis

KW - meiRNA

KW - Nuclear export

KW - Nuclear import

KW - Nucleocytoplasmic shuttling

KW - RNA-binding protein

UR - http://www.scopus.com/inward/record.url?scp=0035933599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035933599&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(01)02567-4

DO - 10.1016/S0014-5793(01)02567-4

M3 - Article

C2 - 11423126

AN - SCOPUS:0035933599

VL - 499

SP - 251

EP - 255

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -