The gene transfer agent-like particle of the marine phototrophic bacterium Rhodovulum sulfidophilum

Nobuyoshi Nagao, Junya Yamamoto, Hiroyuki Komatsu, Hiromichi Suzuki, Yuu Hirose, So Umekage, Takashi Ohyama, Yo Kikuchi

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Gene transfer agents (GTAs) are shaped like bacteriophage particles but have many properties that distinguish them from bacteriophages. GTAs play a role in horizontal gene transfer in nature and thus affect the evolution of prokaryotic genomes. In the course of studies on the extracellular production of designed RNAs using the marine bacterium Rhodovulum sulfidophilum, we found that this bacterium produces a GTA-like particle. The particle contains DNA fragments of 4.5. kb, which consist of randomly fragmented genomic DNA from the bacterium. This 4.5-kb DNA production was prevented while quorum sensing was inhibited. Direct observation of the particle by transmission electron microscopy revealed that the particle resembles a tailed phage and has a head diameter of about 40. nm and a tail length of about 60. nm. We also identified the structural genes for the GTA in the genome. Translated amino acid sequences and gene positions are closely related to those of the genes that encode the Rhodobacter capsulatus GTA. This is the first report of a GTA-like particle from the genus Rhodovulum. However, gene transfer activity of this particle has not yet been confirmed. The differences between this particle and other GTAs are discussed.

    Original languageEnglish
    Pages (from-to)369-374
    Number of pages6
    JournalBiochemistry and Biophysics Reports
    Volume4
    DOIs
    Publication statusPublished - 2015 Dec 1

    Fingerprint

    Rhodovulum
    Gene transfer
    Bacteria
    Genes
    Bacteriophages
    DNA
    Genome
    Rhodobacter capsulatus
    Horizontal Gene Transfer
    Quorum Sensing
    Gene Order
    Transmission Electron Microscopy

    Keywords

    • Artificial RNAs
    • Extracellular nucleic acids
    • Gene transfer
    • Genome sequence
    • Rhodobacter capsulatus

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Molecular Biology

    Cite this

    The gene transfer agent-like particle of the marine phototrophic bacterium Rhodovulum sulfidophilum. / Nagao, Nobuyoshi; Yamamoto, Junya; Komatsu, Hiroyuki; Suzuki, Hiromichi; Hirose, Yuu; Umekage, So; Ohyama, Takashi; Kikuchi, Yo.

    In: Biochemistry and Biophysics Reports, Vol. 4, 01.12.2015, p. 369-374.

    Research output: Contribution to journalArticle

    Nagao, Nobuyoshi ; Yamamoto, Junya ; Komatsu, Hiroyuki ; Suzuki, Hiromichi ; Hirose, Yuu ; Umekage, So ; Ohyama, Takashi ; Kikuchi, Yo. / The gene transfer agent-like particle of the marine phototrophic bacterium Rhodovulum sulfidophilum. In: Biochemistry and Biophysics Reports. 2015 ; Vol. 4. pp. 369-374.
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