The genome landscape of the African Green Monkey kidney-derived vero cell line

Osada Naoki, Kohara Arihiro, Yamaji Toshiyuki, Hirayama Noriko, Kasai Fumio, Sekizuka Suyoshi, Kuroda Makoto, Hanada Kentaro, Masahira Hattori

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals. Verocells aresusceptible to various types of microbes and toxins and have widely contributed to not only microbiology, but also the production of vaccines for human use. We here showed the genome landscape of a Vero cell line, in which 25,877 putative protein-coding genes were identified in the 2.97-Gb genome sequence. A homozygous ∼9-Mb deletion on chromosome 12 caused the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitor genes in Vero cells. In addition, an ∼59-Mb loss of heterozygosity around this deleted region suggested that the homozygosity of the deletion was established by a large-scale conversion. Moreover, a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus. These results revealed the genomic basis for the non-tumourigenic permanent Vero cell lineage susceptible to various pathogens and will be useful for generating new sub-lines and developing new tools in the quality control of Vero cells.

Original languageEnglish
Pages (from-to)673-683
Number of pages11
JournalDNA Research
Volume21
Issue number6
DOIs
Publication statusPublished - 2014 Dec 1
Externally publishedYes

Keywords

  • Animal cell substrate
  • Infectious diseases
  • Vaccine
  • Vero cell
  • Whole genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Naoki, O., Arihiro, K., Toshiyuki, Y., Noriko, H., Fumio, K., Suyoshi, S., Makoto, K., Kentaro, H., & Hattori, M. (2014). The genome landscape of the African Green Monkey kidney-derived vero cell line. DNA Research, 21(6), 673-683. https://doi.org/10.1093/dnares/dsu029