The genome landscape of the African Green Monkey kidney-derived vero cell line

Osada Naoki, Kohara Arihiro, Yamaji Toshiyuki, Hirayama Noriko, Kasai Fumio, Sekizuka Suyoshi, Kuroda Makoto, Hanada Kentaro, Masahira Hattori

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals. Verocells aresusceptible to various types of microbes and toxins and have widely contributed to not only microbiology, but also the production of vaccines for human use. We here showed the genome landscape of a Vero cell line, in which 25,877 putative protein-coding genes were identified in the 2.97-Gb genome sequence. A homozygous ∼9-Mb deletion on chromosome 12 caused the loss of the type I interferon gene cluster and cyclin-dependent kinase inhibitor genes in Vero cells. In addition, an ∼59-Mb loss of heterozygosity around this deleted region suggested that the homozygosity of the deletion was established by a large-scale conversion. Moreover, a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus. These results revealed the genomic basis for the non-tumourigenic permanent Vero cell lineage susceptible to various pathogens and will be useful for generating new sub-lines and developing new tools in the quality control of Vero cells.

Original languageEnglish
Pages (from-to)673-683
Number of pages11
JournalDNA Research
Volume21
Issue number6
DOIs
Publication statusPublished - 2014 Dec 1
Externally publishedYes

Fingerprint

Cercopithecus aethiops
Vero Cells
Genome
Kidney
Cell Line
Simian Retroviruses
Endogenous Retroviruses
Chromosomes, Human, Pair 12
Interferon Type I
Cyclin-Dependent Kinases
Biological Science Disciplines
Loss of Heterozygosity
Cell Lineage
Multigene Family
Microbiology
Quality Control
Vaccines
Pharmaceutical Preparations
Genes
Proteins

Keywords

  • Animal cell substrate
  • Infectious diseases
  • Vaccine
  • Vero cell
  • Whole genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Naoki, O., Arihiro, K., Toshiyuki, Y., Noriko, H., Fumio, K., Suyoshi, S., ... Hattori, M. (2014). The genome landscape of the African Green Monkey kidney-derived vero cell line. DNA Research, 21(6), 673-683. https://doi.org/10.1093/dnares/dsu029

The genome landscape of the African Green Monkey kidney-derived vero cell line. / Naoki, Osada; Arihiro, Kohara; Toshiyuki, Yamaji; Noriko, Hirayama; Fumio, Kasai; Suyoshi, Sekizuka; Makoto, Kuroda; Kentaro, Hanada; Hattori, Masahira.

In: DNA Research, Vol. 21, No. 6, 01.12.2014, p. 673-683.

Research output: Contribution to journalArticle

Naoki, O, Arihiro, K, Toshiyuki, Y, Noriko, H, Fumio, K, Suyoshi, S, Makoto, K, Kentaro, H & Hattori, M 2014, 'The genome landscape of the African Green Monkey kidney-derived vero cell line', DNA Research, vol. 21, no. 6, pp. 673-683. https://doi.org/10.1093/dnares/dsu029
Naoki O, Arihiro K, Toshiyuki Y, Noriko H, Fumio K, Suyoshi S et al. The genome landscape of the African Green Monkey kidney-derived vero cell line. DNA Research. 2014 Dec 1;21(6):673-683. https://doi.org/10.1093/dnares/dsu029
Naoki, Osada ; Arihiro, Kohara ; Toshiyuki, Yamaji ; Noriko, Hirayama ; Fumio, Kasai ; Suyoshi, Sekizuka ; Makoto, Kuroda ; Kentaro, Hanada ; Hattori, Masahira. / The genome landscape of the African Green Monkey kidney-derived vero cell line. In: DNA Research. 2014 ; Vol. 21, No. 6. pp. 673-683.
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