The human actin-related protein hArp5: Nucleo-cytoplasmic shuttling and involvement in DNA repair

Kumiko Kitayama, Mariko Kamo, Yukako Oma, Ryo Matsuda, Takafumi Uchida, Tsuyoshi Ikura, Satoshi Tashiro, Takashi Ohyama, Barbara Winsor, Masahiko Harata

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Certain actin-related proteins (Arps) of budding yeast are localized in the nucleus, and have essential roles as stoichiometric components of histone acetyltransferase (HAT) and chromatin remodeling complexes. On the other hand, identification of vertebrate nuclear Arps and their functional analyses are just beginning. We show that human Arp5 (hArp5) proteins are localized in the nucleus, and that arp5Δ yeast cells are partially complemented by hArp5. Thus, hArp5 is a novel member of the nuclear Arps of vertebrates, which possess evolutionarily conserved functions from yeast to humans. We show here that hArp5 shuttles between the nucleus and the cytoplasm. Furthermore, after the induction of DNA double strand breaks (DSB), cell growth and the accumulation of phosphorylated histone H2AX (γ-H2AX) are impaired by hArp5 depletion. Association of hArp5 with the hIno80 chromatin remodeling enzyme and decrease of chromatin-bound hIno80 by hArp5-depletion indicate that hArp5 may have a role in the recruitment of the hINO80 complex to chromatin. Overexpression of hArp5 and hIno80 enhanced γ-H2AX accumulation. These observations suggest that hArp5 is involved in the process of DSB repair through the regulation of the chromatin remodelling machinery.

    Original languageEnglish
    Pages (from-to)206-217
    Number of pages12
    JournalExperimental Cell Research
    Volume315
    Issue number2
    DOIs
    Publication statusPublished - 2009 Jan 15

    Fingerprint

    DNA Repair
    Actins
    Proteins
    Chromatin Assembly and Disassembly
    Chromatin
    Vertebrates
    Yeasts
    Histone Acetyltransferases
    Saccharomycetales
    Double-Stranded DNA Breaks
    Histones
    Cytoplasm
    Enzymes

    Keywords

    • Actin-related protein
    • Chromatin remodeling
    • DNA repair
    • Genome stability
    • Nucleo-cytoplasmic shuttling
    • Regulatory subunit of complex

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Kitayama, K., Kamo, M., Oma, Y., Matsuda, R., Uchida, T., Ikura, T., ... Harata, M. (2009). The human actin-related protein hArp5: Nucleo-cytoplasmic shuttling and involvement in DNA repair. Experimental Cell Research, 315(2), 206-217. https://doi.org/10.1016/j.yexcr.2008.10.028

    The human actin-related protein hArp5 : Nucleo-cytoplasmic shuttling and involvement in DNA repair. / Kitayama, Kumiko; Kamo, Mariko; Oma, Yukako; Matsuda, Ryo; Uchida, Takafumi; Ikura, Tsuyoshi; Tashiro, Satoshi; Ohyama, Takashi; Winsor, Barbara; Harata, Masahiko.

    In: Experimental Cell Research, Vol. 315, No. 2, 15.01.2009, p. 206-217.

    Research output: Contribution to journalArticle

    Kitayama, K, Kamo, M, Oma, Y, Matsuda, R, Uchida, T, Ikura, T, Tashiro, S, Ohyama, T, Winsor, B & Harata, M 2009, 'The human actin-related protein hArp5: Nucleo-cytoplasmic shuttling and involvement in DNA repair', Experimental Cell Research, vol. 315, no. 2, pp. 206-217. https://doi.org/10.1016/j.yexcr.2008.10.028
    Kitayama, Kumiko ; Kamo, Mariko ; Oma, Yukako ; Matsuda, Ryo ; Uchida, Takafumi ; Ikura, Tsuyoshi ; Tashiro, Satoshi ; Ohyama, Takashi ; Winsor, Barbara ; Harata, Masahiko. / The human actin-related protein hArp5 : Nucleo-cytoplasmic shuttling and involvement in DNA repair. In: Experimental Cell Research. 2009 ; Vol. 315, No. 2. pp. 206-217.
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