Abstract
The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCKBMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by InCLOCK and mBMAL1. (C) Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 224-233 |
| Number of pages | 10 |
| Journal | Genomics |
| Volume | 65 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 May 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
The human and mouse Period1 genes : Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription. / Hida, Akiko; Koike, Nobuya; Hirose, Matsumi; Hattori, Masahira; Sakaki, Yoshiyuki; Tei, Hajime.
In: Genomics, Vol. 65, No. 3, 01.05.2000, p. 224-233.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The human and mouse Period1 genes
T2 - Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription
AU - Hida, Akiko
AU - Koike, Nobuya
AU - Hirose, Matsumi
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuki
AU - Tei, Hajime
PY - 2000/5/1
Y1 - 2000/5/1
N2 - The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCKBMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by InCLOCK and mBMAL1. (C) Academic Press.
AB - The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCKBMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by InCLOCK and mBMAL1. (C) Academic Press.
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U2 - 10.1006/geno.2000.6166
DO - 10.1006/geno.2000.6166
M3 - Article
VL - 65
SP - 224
EP - 233
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -