The influence of artificially introduced nglycosylation sites on the in vitro activity of xenopus laevis erythropoietin

Kazumichi Nagasawa, Mizue Meguro, Kei Sato, Yuta Tanizaki, Nami Nogawa Kosaka, Takashi Kato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Erythropoietin (EPO), the primary regulator of erythropoiesis, is a heavily glycosylated protein found in humans and several other mammals. Intriguingly, we have previously found that EPO in Xenopus laevis (xlEPO) has no N-glycosylation sites, and cross-reacts with the human EPO (huEPO) receptor despite low homology with huEPO. In this study, we introduced N-glycosylation sites into wild-type xlEPO at the positions homologous to those in huEPO, and tested whether the glycosylated mutein retained its biological activity. Seven xlEPO muteins, containing 1-3 additional N-linked carbohydrates at positions 24, 38, and/ or 83, were expressed in COS-1 cells. The muteins exhibited lower secretion efficiency, higher hydrophilicity, and stronger acidic properties than the wild type. All muteins stimulated the proliferation of both cell lines, xlEPO receptor-expressing xlEPOR-FDC/P2 cells and huEPO receptor-expressing UT-7/EPO cells, in a dose-dependent manner. Thus, the muteins retained their in vitro biological activities. The maximum effect on xlEPOR-FDC/P2 proliferation was decreased by the addition of N-linked carbohydrates, but that on UT-7/ EPO proliferation was not changed, indicating that the muteins act as partial agonists to the xlEPO receptor, and near-full agonists to the huEPO receptor. Hence, the EPO-EPOR binding site in X. laevis locates the distal region of artificially introduced three N-glycosylation sites, demonstrating that the vital conformation to exert biological activity is conserved between humans and X. laevis, despite the low similarity in primary structures of EPO and EPOR.

Original languageEnglish
Article numbere0124676
JournalPLoS One
Volume10
Issue number4
DOIs
Publication statusPublished - 2015 Apr 21

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erythropoietin
Xenopus laevis
Erythropoietin
Glycosylation
Bioactivity
glycosylation
receptors
bioactive properties
agonists
Carbohydrates
Erythropoietin Receptors
Mammals
carbohydrates
Hydrophilicity
erythropoiesis
Erythropoiesis
COS Cells
cells
Conformations
In Vitro Techniques

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The influence of artificially introduced nglycosylation sites on the in vitro activity of xenopus laevis erythropoietin. / Nagasawa, Kazumichi; Meguro, Mizue; Sato, Kei; Tanizaki, Yuta; Kosaka, Nami Nogawa; Kato, Takashi.

In: PLoS One, Vol. 10, No. 4, e0124676, 21.04.2015.

Research output: Contribution to journalArticle

Nagasawa, Kazumichi ; Meguro, Mizue ; Sato, Kei ; Tanizaki, Yuta ; Kosaka, Nami Nogawa ; Kato, Takashi. / The influence of artificially introduced nglycosylation sites on the in vitro activity of xenopus laevis erythropoietin. In: PLoS One. 2015 ; Vol. 10, No. 4.
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