The involvement of calmodulin and Ca2+/calmodulin-dependent protein kinase II in the circadian rhythms controlled by the suprachiasmatic nucleus

Tatsuto Fukushima, Takao Shimazoe, Shigenobu Shibata, Akihito Watanabe, Michiko Ono, Toshiyuki Hamada, Shigenori Watanabe

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    We investigated the involvement of calmodulin and Ca2+/calmodulin- dependent protein kinase II (CaMKII) in the photic entrainment of circadian rhythms using calmodulin inhibitors such as calmidazolium (CMZ) and trifluoperazine (TFP), and a CaMKII inhibitor, KN-62, in rats. Fos expression in the suprachiasmatic nucleus (SCN) of rats induced by photic stimulation (300 lux, 1 h) during the early subjective night of the rats was inhibited by treatment with CMZ (10 mg/kg i.p.) or TFP (20 mg/kg i.p.) 30 min before photic stimulation. With respect to the neuronal firing rate in the rat SCN slice, KN-62 and CMZ application during the early subjective night attenuated the glutamate (10 μM)-induced phase shift. The present results suggest that calmodulin and CaMKII are involved in the photic entrainment mechanism in the rodent SCN.

    Original languageEnglish
    Pages (from-to)45-48
    Number of pages4
    JournalNeuroscience Letters
    Volume227
    Issue number1
    DOIs
    Publication statusPublished - 1997 May 9

    Fingerprint

    calmidazolium
    Calcium-Calmodulin-Dependent Protein Kinase Type 2
    Suprachiasmatic Nucleus
    KN 62
    Calmodulin
    Circadian Rhythm
    Trifluoperazine
    Photic Stimulation
    Protein Kinase Inhibitors
    Cell Division
    Glutamic Acid
    Rodentia

    Keywords

    • Ca/calmodulin-dependent protein kinase II
    • Calmodulin
    • Circadian rhythm
    • Photic entrainment
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    The involvement of calmodulin and Ca2+/calmodulin-dependent protein kinase II in the circadian rhythms controlled by the suprachiasmatic nucleus. / Fukushima, Tatsuto; Shimazoe, Takao; Shibata, Shigenobu; Watanabe, Akihito; Ono, Michiko; Hamada, Toshiyuki; Watanabe, Shigenori.

    In: Neuroscience Letters, Vol. 227, No. 1, 09.05.1997, p. 45-48.

    Research output: Contribution to journalArticle

    Fukushima, Tatsuto ; Shimazoe, Takao ; Shibata, Shigenobu ; Watanabe, Akihito ; Ono, Michiko ; Hamada, Toshiyuki ; Watanabe, Shigenori. / The involvement of calmodulin and Ca2+/calmodulin-dependent protein kinase II in the circadian rhythms controlled by the suprachiasmatic nucleus. In: Neuroscience Letters. 1997 ; Vol. 227, No. 1. pp. 45-48.
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