The molecular characterization and in situ expression pattern of pea SCARECROW gene

Naomi Sassa, Yasuhiko Matsushita, Teruko Nakamura, Hiroshi Nyunoya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.

Original languageEnglish
Pages (from-to)385-394
Number of pages10
JournalPlant and Cell Physiology
Volume42
Issue number4
DOIs
Publication statusPublished - 2001 Apr
Externally publishedYes

Fingerprint

Peas
endodermis
peas
Arabidopsis
Genes
shoots
genes
Arabidopsis thaliana
Zea mays
Gravity Sensing
pea protein
Gravitropism
gravitropism
leaf primordia
shoot meristems
asparagine
transcriptional activation
apical meristems
gravity
cDNA libraries

Keywords

  • Endodermis
  • Pea
  • Pisum sativum
  • SCARECROW
  • SCR

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

Cite this

The molecular characterization and in situ expression pattern of pea SCARECROW gene. / Sassa, Naomi; Matsushita, Yasuhiko; Nakamura, Teruko; Nyunoya, Hiroshi.

In: Plant and Cell Physiology, Vol. 42, No. 4, 04.2001, p. 385-394.

Research output: Contribution to journalArticle

Sassa, Naomi ; Matsushita, Yasuhiko ; Nakamura, Teruko ; Nyunoya, Hiroshi. / The molecular characterization and in situ expression pattern of pea SCARECROW gene. In: Plant and Cell Physiology. 2001 ; Vol. 42, No. 4. pp. 385-394.
@article{b53cc991efe74686bcffe764683fb21d,
title = "The molecular characterization and in situ expression pattern of pea SCARECROW gene",
abstract = "Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.",
keywords = "Endodermis, Pea, Pisum sativum, SCARECROW, SCR",
author = "Naomi Sassa and Yasuhiko Matsushita and Teruko Nakamura and Hiroshi Nyunoya",
year = "2001",
month = "4",
doi = "10.1093/pcp/pce048",
language = "English",
volume = "42",
pages = "385--394",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - The molecular characterization and in situ expression pattern of pea SCARECROW gene

AU - Sassa, Naomi

AU - Matsushita, Yasuhiko

AU - Nakamura, Teruko

AU - Nyunoya, Hiroshi

PY - 2001/4

Y1 - 2001/4

N2 - Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.

AB - Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.

KW - Endodermis

KW - Pea

KW - Pisum sativum

KW - SCARECROW

KW - SCR

UR - http://www.scopus.com/inward/record.url?scp=0035022334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035022334&partnerID=8YFLogxK

U2 - 10.1093/pcp/pce048

DO - 10.1093/pcp/pce048

M3 - Article

C2 - 11333309

AN - SCOPUS:0035022334

VL - 42

SP - 385

EP - 394

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 4

ER -