The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles

Akihiro Hiraguri, Hiroyuki Hibino, Takaharu Hayashi, Osamu Netsu, Takumi Shimizu, Tamaki Uehara-Ichiki, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Gene 3 in the genomes of several plant-infecting rhabdoviruses, including rice transitory yellowing virus (RTYV), has been postulated to encode a cell-to-cell movement protein (MP). Transcomplementation experiments using a movement-defective tomato mosaic virus and the P3 protein of RTYV, encoded by gene 3, facilitated intercellular transport of the mutant virus. In transient-expression experiments with the GFP-fused P3 protein in epidermal leaf cells of Nicotiana benthamiana, the P3 protein was associated with the nucleus and plasmodesmata. Immunogold-labelling studies of thin sections of RTYV-infected rice plants using an antiserum against Escherichia coli-expressed His6-tagged P3 protein indicated that the P3 protein was located in cell walls and on virus particles. In Western blots using antisera against E. coliexpressed P3 protein and purified RTYV, the P3 protein was detected in purified RTYV, whilst antiserum against purified RTYV reacted with the E. coli-expressed P3 protein. After immunogold labelling of crude sap from RTYV-infected rice leaves, the P3 protein, as well as the N protein, was detected on the ribonucleocapsid core that emerged from partially disrupted virus particles. These results provide evidence that the P3 protein of RTYV, which functions as a viral MP, is a viral structural protein and seems to be associated with the ribonucleocapsid core of virus particles.

Original languageEnglish
Pages (from-to)2290-2298
Number of pages9
JournalJournal of General Virology
Volume93
Issue numberPART 10
DOIs
Publication statusPublished - 2012 Oct
Externally publishedYes

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Satellite Viruses
Virion
Viruses
Proteins
Immune Sera
Oryza
Plasmodesmata
Rhabdoviridae
Escherichia coli
Viral Structural Proteins
Plant Genome
Mosaic Viruses
Viral Proteins
Lycopersicon esculentum
Cell Wall
Genes
Tobacco
Cell Movement

ASJC Scopus subject areas

  • Virology

Cite this

Hiraguri, A., Hibino, H., Hayashi, T., Netsu, O., Shimizu, T., Uehara-Ichiki, T., ... Sasaya, T. (2012). The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles. Journal of General Virology, 93(PART 10), 2290-2298. https://doi.org/10.1099/vir.0.044420-0

The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles. / Hiraguri, Akihiro; Hibino, Hiroyuki; Hayashi, Takaharu; Netsu, Osamu; Shimizu, Takumi; Uehara-Ichiki, Tamaki; Omura, Toshihiro; Sasaki, Nobumitsu; Nyunoya, Hiroshi; Sasaya, Takahide.

In: Journal of General Virology, Vol. 93, No. PART 10, 10.2012, p. 2290-2298.

Research output: Contribution to journalArticle

Hiraguri, A, Hibino, H, Hayashi, T, Netsu, O, Shimizu, T, Uehara-Ichiki, T, Omura, T, Sasaki, N, Nyunoya, H & Sasaya, T 2012, 'The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles', Journal of General Virology, vol. 93, no. PART 10, pp. 2290-2298. https://doi.org/10.1099/vir.0.044420-0
Hiraguri, Akihiro ; Hibino, Hiroyuki ; Hayashi, Takaharu ; Netsu, Osamu ; Shimizu, Takumi ; Uehara-Ichiki, Tamaki ; Omura, Toshihiro ; Sasaki, Nobumitsu ; Nyunoya, Hiroshi ; Sasaya, Takahide. / The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles. In: Journal of General Virology. 2012 ; Vol. 93, No. PART 10. pp. 2290-2298.
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abstract = "Gene 3 in the genomes of several plant-infecting rhabdoviruses, including rice transitory yellowing virus (RTYV), has been postulated to encode a cell-to-cell movement protein (MP). Transcomplementation experiments using a movement-defective tomato mosaic virus and the P3 protein of RTYV, encoded by gene 3, facilitated intercellular transport of the mutant virus. In transient-expression experiments with the GFP-fused P3 protein in epidermal leaf cells of Nicotiana benthamiana, the P3 protein was associated with the nucleus and plasmodesmata. Immunogold-labelling studies of thin sections of RTYV-infected rice plants using an antiserum against Escherichia coli-expressed His6-tagged P3 protein indicated that the P3 protein was located in cell walls and on virus particles. In Western blots using antisera against E. coliexpressed P3 protein and purified RTYV, the P3 protein was detected in purified RTYV, whilst antiserum against purified RTYV reacted with the E. coli-expressed P3 protein. After immunogold labelling of crude sap from RTYV-infected rice leaves, the P3 protein, as well as the N protein, was detected on the ribonucleocapsid core that emerged from partially disrupted virus particles. These results provide evidence that the P3 protein of RTYV, which functions as a viral MP, is a viral structural protein and seems to be associated with the ribonucleocapsid core of virus particles.",
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