The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

Tomoaki Ando, Terumichi Tanaka, Yo Kikuchi

Research output: Contribution to journalArticle


We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.

Original languageEnglish
Pages (from-to)1782-1785
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number8
Publication statusPublished - 2004 Aug
Externally publishedYes



  • Escherichia coli
  • Hyperprocessing
  • Ribonuclease P
  • Tyrosine transfer RNA (tRNA)

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

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