The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos

Virginie Hachet, Coralie Busso, Mika Toya, Asako Sugimoto, Peter Askjaer, Pierre Gönczy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Regulation of mitosis in time and space is critical for proper cell division. We conducted an RNA interference-based modifier screen to identify novel regulators of mitosis in Caenorhabditis elegans embryos. Of particular interest, this screen revealed that the Nup205 nucleoporin NPP-3 can negatively modulate the timing of mitotic onset. Furthermore, we discovered that NPP-3 and nucleoporins that are associated with it are lost from the nuclear envelope (NE) in the vicinity of centrosomes at the onset of mitosis. We demonstrate that centrosomes are both necessary and sufficient for NPP-3 local loss, which also requires the activity of the Aurora-A kinase AIR-1. Our findings taken together support a model in which centrosomes and AIR-1 promote timely onset of mitosis by locally removing NPP-3 and associated nucleoporins from the NE.

Original languageEnglish
Pages (from-to)3111-3121
Number of pages11
JournalMolecular Biology of the Cell
Volume23
Issue number16
DOIs
Publication statusPublished - 2012 Aug 15
Externally publishedYes

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Nuclear Pore Complex Proteins
Centrosome
Caenorhabditis elegans
Mitosis
Embryonic Structures
Nuclear Envelope
Aurora Kinase A
RNA Interference
Cell Division

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos. / Hachet, Virginie; Busso, Coralie; Toya, Mika; Sugimoto, Asako; Askjaer, Peter; Gönczy, Pierre.

In: Molecular Biology of the Cell, Vol. 23, No. 16, 15.08.2012, p. 3111-3121.

Research output: Contribution to journalArticle

Hachet, Virginie ; Busso, Coralie ; Toya, Mika ; Sugimoto, Asako ; Askjaer, Peter ; Gönczy, Pierre. / The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos. In: Molecular Biology of the Cell. 2012 ; Vol. 23, No. 16. pp. 3111-3121.
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