The Optical Activity of Lysozyme Crystals

J. Kobayashi, Toru Asahi, M. Sakurai, I. Kagomiya, H. Asai, H. Asami

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    The components of the gyration tensor of the enzyme lysozyme were measured by using the HAUP method: g11 = - 0.90 × 10-5 and g33 = 1.05 × 10-5 at 303.4 K and a wavelength of 4880 Å. The optical rotatory powers along the a and c axes in the same conditions were calculated: ρa = -21.3 and ρc. = -24.8° cm-1. The optically active property of lysozyme is strange in that, although it contains a considerable quantity of α-helices (about 30%), the rotatory powers are unexpectedly small in magnitude, one order of magnitude less than those of quartz and with very large anisotropy. A conceptual consideration of this phenomenon is given. In order to assess the difference between the structures in both crystalline and solution states, the chirality index r was calculated to be 0.16. This value indicates that the structural change of lysozyme from the solution into the crystalline state is expressed by an increase of 19% in optical activity. From the NMR results [Smith et al. (1993), J. Mol. Biol. 229, 930-944], it is anticipated that the r value reflects the increased constraint in atomic motion in the side chains of exposed amino acid residues in the crystalline state.

    Original languageEnglish
    Pages (from-to)581-590
    Number of pages10
    JournalActa Crystallographica Section A: Foundations of Crystallography
    Volume54
    Issue number5
    Publication statusPublished - 1998 Sep 1

    Fingerprint

    Optical Rotation
    optical activity
    lysozyme
    Muramidase
    Enzymes
    Crystalline materials
    Crystals
    crystals
    Quartz
    Chirality
    Anisotropy
    gyration
    chirality
    helices
    Tensors
    amino acids
    enzymes
    quartz
    Nuclear magnetic resonance
    tensors

    ASJC Scopus subject areas

    • Structural Biology
    • Condensed Matter Physics

    Cite this

    Kobayashi, J., Asahi, T., Sakurai, M., Kagomiya, I., Asai, H., & Asami, H. (1998). The Optical Activity of Lysozyme Crystals. Acta Crystallographica Section A: Foundations of Crystallography, 54(5), 581-590.

    The Optical Activity of Lysozyme Crystals. / Kobayashi, J.; Asahi, Toru; Sakurai, M.; Kagomiya, I.; Asai, H.; Asami, H.

    In: Acta Crystallographica Section A: Foundations of Crystallography, Vol. 54, No. 5, 01.09.1998, p. 581-590.

    Research output: Contribution to journalArticle

    Kobayashi, J, Asahi, T, Sakurai, M, Kagomiya, I, Asai, H & Asami, H 1998, 'The Optical Activity of Lysozyme Crystals', Acta Crystallographica Section A: Foundations of Crystallography, vol. 54, no. 5, pp. 581-590.
    Kobayashi J, Asahi T, Sakurai M, Kagomiya I, Asai H, Asami H. The Optical Activity of Lysozyme Crystals. Acta Crystallographica Section A: Foundations of Crystallography. 1998 Sep 1;54(5):581-590.
    Kobayashi, J. ; Asahi, Toru ; Sakurai, M. ; Kagomiya, I. ; Asai, H. ; Asami, H. / The Optical Activity of Lysozyme Crystals. In: Acta Crystallographica Section A: Foundations of Crystallography. 1998 ; Vol. 54, No. 5. pp. 581-590.
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