The 'polysemous' codon - A codon with multiple amino acid assignment caused by dual specificity of tRNA identity

Tsutomu Suzuki, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

In some Candida species, the universal CUG leucine codon is translated as serine. However, in most cases, the serine tRNAs responsible for this non-universal decoding (tRNA(Ser)CAG) accept in vitro not only serine, but also, to some extent, leucine. Nucleotide replacement experiments indicated that m1G37 is critical for leucylation activity. This finding was supported by the fact that the tRNA(Ser)CAGs possessing the leucylation activity always have m1G37, whereas that of Candida cylindracea, which possesses no leucylation activity, has A37. Quantification of defined aminoacetylated tRNAs in cells demonstrated that 3% of the tRNA(Ser)CAGs possessing m1G37 were, in fact, charged with leucine in vivo. A genetic approach using an auxotroph mutant of C. maltosa possessing this type of tRNA(Ser)CAG also suggested that the URA3 gene inactivated due to the translation of CUG as serine was rescued by a slight incorporation of leucine into the polypeptide, which demonstrated that the tRNA charged with multiple amino acids could participate in the translation. These findings provide the first evidence that two distinct amino acids are assigned by a single codon, which occurs naturally in the translation process of certain Candida species. We term this novel type of codon a 'polysemous codon'.

Original languageEnglish
Pages (from-to)1122-1134
Number of pages13
JournalEMBO Journal
Volume16
Issue number5
DOIs
Publication statusPublished - 1997 Mar 3

Keywords

  • Aminoacyl-tRNA synthetase
  • Candida
  • Genetic code
  • Translational fidelity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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