The potential of stem cells in adult tissues representative of the three germ layers

Haruko Obokata, Koji Kojima, Karen Westerman, Masayuki Yamato, Teruo Okano, Satoshi Tsuneda, Charles A. Vacanti

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Mature adult tissues contain stem cells that express many genes normally associated with the early stage of embryonic development, when maintained in appropriate environments. Cells procured from adult tissues representative of the three germ layers (spinal cord, muscle, and lung), each exhibiting the potential to mature into cells representative of all three germ layers. Cells isolated from adult tissues of different germ layer origin were propagated as nonadherent clusters or spheres that were composed of heterogeneous populations of cells. When the clusters or spheres were dissociated, the cells had the ability to reform new, nonadherent spheres for several generations. When implanted in vivo, in association with biodegradable scaffolds, into immunodeficient mice, tissue containing cells characteristic of the three germ layers was generated. These findings suggest the existence of a population of stem cells in adult tissues that is quite different and distinct from embryonic stem cells that demonstrate a greater potency for differentiation across germ lines than previously believed. Such cells could potentially be as useful as embryonic stem cells in tissue engineering and regenerative medicine.

    Original languageEnglish
    Pages (from-to)607-615
    Number of pages9
    JournalTissue Engineering - Part A
    Volume17
    Issue number5-6
    DOIs
    Publication statusPublished - 2011 Mar 1

    Fingerprint

    Germ Layers
    Adult Stem Cells
    Stem cells
    Tissue
    Embryonic Stem Cells
    Tissue engineering
    Scaffolds
    Muscle
    Regenerative Medicine
    Genes
    Tissue Engineering
    Cells
    Germ Cells
    Population
    Embryonic Development
    Spinal Cord
    Stem Cells
    Muscles
    Lung

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Biomedical Engineering
    • Biomaterials
    • Medicine(all)

    Cite this

    Obokata, H., Kojima, K., Westerman, K., Yamato, M., Okano, T., Tsuneda, S., & Vacanti, C. A. (2011). The potential of stem cells in adult tissues representative of the three germ layers. Tissue Engineering - Part A, 17(5-6), 607-615. https://doi.org/10.1089/ten.tea.2010.0385

    The potential of stem cells in adult tissues representative of the three germ layers. / Obokata, Haruko; Kojima, Koji; Westerman, Karen; Yamato, Masayuki; Okano, Teruo; Tsuneda, Satoshi; Vacanti, Charles A.

    In: Tissue Engineering - Part A, Vol. 17, No. 5-6, 01.03.2011, p. 607-615.

    Research output: Contribution to journalArticle

    Obokata, H, Kojima, K, Westerman, K, Yamato, M, Okano, T, Tsuneda, S & Vacanti, CA 2011, 'The potential of stem cells in adult tissues representative of the three germ layers', Tissue Engineering - Part A, vol. 17, no. 5-6, pp. 607-615. https://doi.org/10.1089/ten.tea.2010.0385
    Obokata, Haruko ; Kojima, Koji ; Westerman, Karen ; Yamato, Masayuki ; Okano, Teruo ; Tsuneda, Satoshi ; Vacanti, Charles A. / The potential of stem cells in adult tissues representative of the three germ layers. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 5-6. pp. 607-615.
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