The primary structure of iodopsin, a chicken red-sensitive cone pigment

Osamu Kuwata, Yasushi Imamoto, Toshiyuki Okano, Koichi Kokame, Daisuke Kojima, Hiroki Matsumoto, Akihiro Morodome, Yoshitaka Fukada, Yoshinori Shichida, Kunio Yasuda, Yoshiro Shimura, Tôru Yoshizawa

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

A purified iodopsin was digested by CNBr or several proteolytic enzymes into fragments, the amino acid sequences of which were determined. A partial sequence of the C-terminal fragment was utilized for synthesizing an oligonucleotide probe which identified the iodopsin cDNA (1339 bases). The deduced amino acid sequence (362 residues) had 80%, 42% or 43% homology to that of human red-sensitive cone pigment, cattle or chicken rhodospin, respectively. Although the hydropathy profile implies that iodopsin, like rhodopsin, has 7 transmembrane α-helical segments, iodopsin may have a hydrophilic pocket near the seventh segment on the basis of the unexpected cleavages in the middle of the segment VII by chymotrypsin under nondenaturing conditions.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalFEBS Letters
Volume272
Issue number1-2
DOIs
Publication statusPublished - 1990 Oct 15
Externally publishedYes

Fingerprint

Pigments
Cones
Chickens
Amino Acid Sequence
Amino Acids
Rhodopsin
Oligonucleotide Probes
Chymotrypsin
Peptide Hydrolases
Complementary DNA
iodopsin

Keywords

  • cDNA cloning
  • Chicken retina
  • Color vision
  • Cone visual pigment
  • Iodopsin
  • Primary structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kuwata, O., Imamoto, Y., Okano, T., Kokame, K., Kojima, D., Matsumoto, H., ... Yoshizawa, T. (1990). The primary structure of iodopsin, a chicken red-sensitive cone pigment. FEBS Letters, 272(1-2), 128-132. https://doi.org/10.1016/0014-5793(90)80465-U

The primary structure of iodopsin, a chicken red-sensitive cone pigment. / Kuwata, Osamu; Imamoto, Yasushi; Okano, Toshiyuki; Kokame, Koichi; Kojima, Daisuke; Matsumoto, Hiroki; Morodome, Akihiro; Fukada, Yoshitaka; Shichida, Yoshinori; Yasuda, Kunio; Shimura, Yoshiro; Yoshizawa, Tôru.

In: FEBS Letters, Vol. 272, No. 1-2, 15.10.1990, p. 128-132.

Research output: Contribution to journalArticle

Kuwata, O, Imamoto, Y, Okano, T, Kokame, K, Kojima, D, Matsumoto, H, Morodome, A, Fukada, Y, Shichida, Y, Yasuda, K, Shimura, Y & Yoshizawa, T 1990, 'The primary structure of iodopsin, a chicken red-sensitive cone pigment', FEBS Letters, vol. 272, no. 1-2, pp. 128-132. https://doi.org/10.1016/0014-5793(90)80465-U
Kuwata O, Imamoto Y, Okano T, Kokame K, Kojima D, Matsumoto H et al. The primary structure of iodopsin, a chicken red-sensitive cone pigment. FEBS Letters. 1990 Oct 15;272(1-2):128-132. https://doi.org/10.1016/0014-5793(90)80465-U
Kuwata, Osamu ; Imamoto, Yasushi ; Okano, Toshiyuki ; Kokame, Koichi ; Kojima, Daisuke ; Matsumoto, Hiroki ; Morodome, Akihiro ; Fukada, Yoshitaka ; Shichida, Yoshinori ; Yasuda, Kunio ; Shimura, Yoshiro ; Yoshizawa, Tôru. / The primary structure of iodopsin, a chicken red-sensitive cone pigment. In: FEBS Letters. 1990 ; Vol. 272, No. 1-2. pp. 128-132.
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