The protein tyrosine kinase Fyn activates transcription from the HIV promoter via activation of NFkB-like DNA-binding proteins

Naohiro Hohashi, Takuma Hayashi, Noemi Fusaki, Masakazu Takeuchi, Makoto Higurashi, Takashi Okamoto, Kentaro Semba, Tadashi Yamamoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Protein tyrosine kinase p59fyn (Fyn) associates with the TCR-CD3 complex, which suggests that Fyn plays a significant role in the signal transduction involving TCR complex. In addition to cellular genes, viral promoters such as the HIV long terminal repeat (LTR) are also activated upon T cell activation. To elucidate the functional significance of Fyn in the expression of viral promoters, we transfected a Fyn-expression vector together with a reporter plasmid containing the chloramphenicol acetyltransferase gene driven by HIV LTR into a human T cell line, Jurkat. In this assay, Fyn stimulated the promoter in HIV LTR when the transfected cells were treated with both concanavalin A and PMA as an antigen-mimic stimulation. This activation required the intact SH2 domain of Fyn. Mutational analysis of HIV LTR showed that the NFKB binding sites were responsible for this effect. Electrophoretic mobility shift assays and UV cross-linking experiments showed that activation of T cells by anti-CD3 antibody induced four KB-binding proteins (50, 60, 65 and 100 kDa) in Fyn-overexpressing cells more efficiently than in the parental cells. Our results suggested that Fyn was able to regulate expression of a subset of genes via KB-binding proteins upon T cell activation.

Original languageEnglish
Pages (from-to)1851-1859
Number of pages9
JournalInternational Immunology
Volume7
Issue number11
DOIs
Publication statusPublished - 1995 Nov 1
Externally publishedYes

Keywords

  • Signal transduction
  • Src family
  • Transcription factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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