The PURE system for protein production

Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (Scopus)

Abstract

In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies.

Original languageEnglish
Title of host publicationCell-Free Protein Synthesis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages275-284
Number of pages10
ISBN (Print)9781627037815
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1118
ISSN (Print)1064-3745

Fingerprint

Cell-Free System
Proteins
Recombinant Proteins
Biochemistry
Molecular Biology
Amino Acid Sequence
Complementary DNA

Keywords

  • Cell-free protein synthesis system
  • In vitro translation system
  • PURE system
  • Ribosome
  • tRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shimizu, Y., Kuruma, Y., Kanamori, T., & Ueda, T. (2014). The PURE system for protein production. In Cell-Free Protein Synthesis: Methods and Protocols (pp. 275-284). (Methods in Molecular Biology; Vol. 1118). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-782-2_19

The PURE system for protein production. / Shimizu, Yoshihiro; Kuruma, Yutetsu; Kanamori, Takashi; Ueda, Takuya.

Cell-Free Protein Synthesis: Methods and Protocols. Humana Press Inc., 2014. p. 275-284 (Methods in Molecular Biology; Vol. 1118).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shimizu, Y, Kuruma, Y, Kanamori, T & Ueda, T 2014, The PURE system for protein production. in Cell-Free Protein Synthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1118, Humana Press Inc., pp. 275-284. https://doi.org/10.1007/978-1-62703-782-2_19
Shimizu Y, Kuruma Y, Kanamori T, Ueda T. The PURE system for protein production. In Cell-Free Protein Synthesis: Methods and Protocols. Humana Press Inc. 2014. p. 275-284. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-782-2_19
Shimizu, Yoshihiro ; Kuruma, Yutetsu ; Kanamori, Takashi ; Ueda, Takuya. / The PURE system for protein production. Cell-Free Protein Synthesis: Methods and Protocols. Humana Press Inc., 2014. pp. 275-284 (Methods in Molecular Biology).
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