The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo

John A. Gaynes, Hideo Otsuna, Douglas Simon Campbell, John P. Manfredi, Edward M. Levine, Chi Bin Chien

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Attractive growth cone turning requires Igf2bp1-dependent local translation of β-actin mRNA in response to external cues in vitro. While in vivo studies have shown that Igf2bp1 is required for cell migration and axon terminal branching, a requirement for Igf2bp1 function during axon outgrowth has not been demonstrated. Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the β-actin 3'UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. Igf2bp1 knockdown reduced RGC axonal outgrowth and tectal coverage and retinal cell survival. RGC-specific expression of a phosphomimetic Igf2bp1 reduced the density of axonal projections in the optic tract while sparing RGCs, demonstrating for the first time that Igf2bp1 is required during axon outgrowth in vivo. Therefore, regulation of local translation mediated by Igf2bp proteins may be required at all stages of axon development.

Original languageEnglish
Article numbere0134751
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1
Externally publishedYes

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RNA-binding proteins
RNA-Binding Proteins
Retinal Ganglion Cells
Zebrafish
Danio rerio
axons
Growth Cones
cones (retina)
actin
Cones
Actins
cells
Presynaptic Terminals
Protein Biosynthesis
3' Untranslated Regions
3' untranslated regions
in vivo studies
optics
cell movement
Cell Movement

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gaynes, J. A., Otsuna, H., Campbell, D. S., Manfredi, J. P., Levine, E. M., & Chien, C. B. (2015). The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo. PLoS One, 10(9), [e0134751]. https://doi.org/10.1371/journal.pone.0134751

The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo. / Gaynes, John A.; Otsuna, Hideo; Campbell, Douglas Simon; Manfredi, John P.; Levine, Edward M.; Chien, Chi Bin.

In: PLoS One, Vol. 10, No. 9, e0134751, 01.09.2015.

Research output: Contribution to journalArticle

Gaynes, JA, Otsuna, H, Campbell, DS, Manfredi, JP, Levine, EM & Chien, CB 2015, 'The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo', PLoS One, vol. 10, no. 9, e0134751. https://doi.org/10.1371/journal.pone.0134751
Gaynes JA, Otsuna H, Campbell DS, Manfredi JP, Levine EM, Chien CB. The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo. PLoS One. 2015 Sep 1;10(9). e0134751. https://doi.org/10.1371/journal.pone.0134751
Gaynes, John A. ; Otsuna, Hideo ; Campbell, Douglas Simon ; Manfredi, John P. ; Levine, Edward M. ; Chien, Chi Bin. / The RNA binding protein Igf2bp1 is required for zebrafish RGC axon outgrowth in vivo. In: PLoS One. 2015 ; Vol. 10, No. 9.
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