The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro

Toshiyuki Hamada, Shigenobu Shibata

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Gamma-aminobutyric acid (GABA), and its biosynthetic enzyme, glutamic decarboxylase, are widely distributed in the suprachiasmatic nucleus (SCN). In the present study, we examined the role of the GABAA receptor on in vitro SCN responses to photic-like signals. We found that 100 μM GABAA receptor antagonist bicuculline partially blocked field potentials evoked by optic nerve stimulation. NMDA- and SP-induced phase shifts of SCN neuronal activity rhythms, were blocked with 10 μM bicuculline. Application of 100 μM bicuculline alone induced phase advance of SCN neuronal activity rhythm. These results show that NMDA- and SP-induced phase shifts are blocked by bicuculline and suggest GABA has an important role as neurotransmitter in the neuronal network regulating phase shifts of the circadian clock.

    Original languageEnglish
    Pages (from-to)344-347
    Number of pages4
    JournalNeuroscience Letters
    Volume468
    Issue number3
    DOIs
    Publication statusPublished - 2010 Jan 14

    Fingerprint

    GABAergic Neurons
    Suprachiasmatic Nucleus
    Bicuculline
    N-Methylaspartate
    gamma-Aminobutyric Acid
    GABA-A Receptor Antagonists
    Circadian Clocks
    Carboxy-Lyases
    GABA-A Receptors
    Optic Nerve
    Evoked Potentials
    Neurotransmitter Agents
    In Vitro Techniques
    Enzymes

    Keywords

    • Bicuculline
    • Circadian rhythm
    • Field potential
    • GABA
    • SCN firing rhythm
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro. / Hamada, Toshiyuki; Shibata, Shigenobu.

    In: Neuroscience Letters, Vol. 468, No. 3, 14.01.2010, p. 344-347.

    Research output: Contribution to journalArticle

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