The Role of Phosvitin for Nucleation of Calcium Phosphates on Collagen

Naoko Kobayashi, Kazuo Onuma, Ayako Oyane, Atsushi Yamazaki

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The role of phospho-protein in bone formation was investigated in this study. A phosvitin layer was formed on a collagen sheet by soaking the sheet in a fluid containing phosvitin of 0.04wt% and various concentrations of dimethylsuberimidate. The dimethylsuberimidate concentration was changed from 0.04 to 0.3wt% to find the appropriate concentration for phosvitin-collagen layer formation. The composite layer was soaked in physiological solutions and the nucleation process of calcium phosphates was observed by using atomic force microscopy (AFM). It was found that small hydroxyapatite (HAP) crystals were formed throughout the layer with their a-axes arranged perpendicular to it. On the other hand, HAP with its c-axis perpendicular to the layer was formed when phosvitin was not present. This makes it clear that the roles of phosvitin are to enhance nucleation of HAP, and to control the orientation of HAP crystals on collagen in the same way as that observed in human bone.

    Original languageEnglish
    Pages (from-to)537-540
    Number of pages4
    JournalKey Engineering Materials
    Volume254-256
    Publication statusPublished - 2004

    Fingerprint

    Phosvitin
    Calcium Phosphates
    Calcium phosphate
    Hydroxyapatite
    Collagen
    Durapatite
    Nucleation
    Dimethyl Suberimidate
    Bone
    Crystals
    Crystal orientation
    Atomic force microscopy
    Proteins
    Fluids
    Composite materials

    Keywords

    • AFM
    • Calcium phosphate
    • Collagen
    • Phosvitin

    ASJC Scopus subject areas

    • Ceramics and Composites
    • Chemical Engineering (miscellaneous)

    Cite this

    The Role of Phosvitin for Nucleation of Calcium Phosphates on Collagen. / Kobayashi, Naoko; Onuma, Kazuo; Oyane, Ayako; Yamazaki, Atsushi.

    In: Key Engineering Materials, Vol. 254-256, 2004, p. 537-540.

    Research output: Contribution to journalArticle

    Kobayashi, Naoko ; Onuma, Kazuo ; Oyane, Ayako ; Yamazaki, Atsushi. / The Role of Phosvitin for Nucleation of Calcium Phosphates on Collagen. In: Key Engineering Materials. 2004 ; Vol. 254-256. pp. 537-540.
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