The role of plant villin in the organization of the actin cytoskeleton, cytoplasmic streaming and the architecture of the transvacuolar strand in root hair cells of Hydrochraris

Motoki Tominaga, Etsuo Yokota, Luis Vidali, Seiji Sonobe, Peter K. Hepler, Teruo Shimmen

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

In many types of plant cell, bundles of actin filaments (AFs) are generally involved in cytoplasmic streaming and the organization of transvacuolar strands. Actin cross-linking proteins are believed to arrange AFs into the bundles. In root hair cells of Hydrocharis dubia (Blume) Baker, a 135-kDa polypeptide cross-reacted with an antiserum against a 135-kDa actin-bundling protein (135-ABP), a villin homologue, isolated from lily pollen tubes. Immunofluorescence microscopy revealed that the 135-kDa polypeptide co-localized with AF bundles in the transvacuolar strand and in the subcortical region of the cells. Microinjection of antiserum against 135-ABP into living root hair cells induced the disappearance of the transvacuolar strand. Concomitantly, thick AF bundles in the transvacuolar strand dispersed into thin bundles. In the root hair cells, AFs showed uniform polarity in the bundles, which is consistent with the in-vitro activity of 135-ABP. These results suggest that villin is a factor responsible for bundling AFs in root hair cells as well as in pollen tubes, and that it plays a key role in determining the direction of cytoplasmic streaming in these cells.

Original languageEnglish
Pages (from-to)836-843
Number of pages8
JournalPlanta
Volume210
Issue number5
Publication statusPublished - 2000 Apr
Externally publishedYes

Fingerprint

Cytoplasmic Streaming
cytoplasmic streaming
root hairs
microfilaments
Actin Cytoskeleton
Actins
actin
Pollen Tube
cells
pollen tubes
Immune Sera
Proteins
antiserum
polypeptides
Lilium
proteins
Peptides
Plant Cells
Microinjections
Fluorescence Microscopy

Keywords

  • Actin bundling protein
  • Actin filament
  • Hydrocharis
  • Root hair cell
  • Transvacuolar strand
  • Villin

ASJC Scopus subject areas

  • Plant Science

Cite this

The role of plant villin in the organization of the actin cytoskeleton, cytoplasmic streaming and the architecture of the transvacuolar strand in root hair cells of Hydrochraris. / Tominaga, Motoki; Yokota, Etsuo; Vidali, Luis; Sonobe, Seiji; Hepler, Peter K.; Shimmen, Teruo.

In: Planta, Vol. 210, No. 5, 04.2000, p. 836-843.

Research output: Contribution to journalArticle

@article{ab19ee5d69b64909a8c5308007c807e2,
title = "The role of plant villin in the organization of the actin cytoskeleton, cytoplasmic streaming and the architecture of the transvacuolar strand in root hair cells of Hydrochraris",
abstract = "In many types of plant cell, bundles of actin filaments (AFs) are generally involved in cytoplasmic streaming and the organization of transvacuolar strands. Actin cross-linking proteins are believed to arrange AFs into the bundles. In root hair cells of Hydrocharis dubia (Blume) Baker, a 135-kDa polypeptide cross-reacted with an antiserum against a 135-kDa actin-bundling protein (135-ABP), a villin homologue, isolated from lily pollen tubes. Immunofluorescence microscopy revealed that the 135-kDa polypeptide co-localized with AF bundles in the transvacuolar strand and in the subcortical region of the cells. Microinjection of antiserum against 135-ABP into living root hair cells induced the disappearance of the transvacuolar strand. Concomitantly, thick AF bundles in the transvacuolar strand dispersed into thin bundles. In the root hair cells, AFs showed uniform polarity in the bundles, which is consistent with the in-vitro activity of 135-ABP. These results suggest that villin is a factor responsible for bundling AFs in root hair cells as well as in pollen tubes, and that it plays a key role in determining the direction of cytoplasmic streaming in these cells.",
keywords = "Actin bundling protein, Actin filament, Hydrocharis, Root hair cell, Transvacuolar strand, Villin",
author = "Motoki Tominaga and Etsuo Yokota and Luis Vidali and Seiji Sonobe and Hepler, {Peter K.} and Teruo Shimmen",
year = "2000",
month = "4",
language = "English",
volume = "210",
pages = "836--843",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - The role of plant villin in the organization of the actin cytoskeleton, cytoplasmic streaming and the architecture of the transvacuolar strand in root hair cells of Hydrochraris

AU - Tominaga, Motoki

AU - Yokota, Etsuo

AU - Vidali, Luis

AU - Sonobe, Seiji

AU - Hepler, Peter K.

AU - Shimmen, Teruo

PY - 2000/4

Y1 - 2000/4

N2 - In many types of plant cell, bundles of actin filaments (AFs) are generally involved in cytoplasmic streaming and the organization of transvacuolar strands. Actin cross-linking proteins are believed to arrange AFs into the bundles. In root hair cells of Hydrocharis dubia (Blume) Baker, a 135-kDa polypeptide cross-reacted with an antiserum against a 135-kDa actin-bundling protein (135-ABP), a villin homologue, isolated from lily pollen tubes. Immunofluorescence microscopy revealed that the 135-kDa polypeptide co-localized with AF bundles in the transvacuolar strand and in the subcortical region of the cells. Microinjection of antiserum against 135-ABP into living root hair cells induced the disappearance of the transvacuolar strand. Concomitantly, thick AF bundles in the transvacuolar strand dispersed into thin bundles. In the root hair cells, AFs showed uniform polarity in the bundles, which is consistent with the in-vitro activity of 135-ABP. These results suggest that villin is a factor responsible for bundling AFs in root hair cells as well as in pollen tubes, and that it plays a key role in determining the direction of cytoplasmic streaming in these cells.

AB - In many types of plant cell, bundles of actin filaments (AFs) are generally involved in cytoplasmic streaming and the organization of transvacuolar strands. Actin cross-linking proteins are believed to arrange AFs into the bundles. In root hair cells of Hydrocharis dubia (Blume) Baker, a 135-kDa polypeptide cross-reacted with an antiserum against a 135-kDa actin-bundling protein (135-ABP), a villin homologue, isolated from lily pollen tubes. Immunofluorescence microscopy revealed that the 135-kDa polypeptide co-localized with AF bundles in the transvacuolar strand and in the subcortical region of the cells. Microinjection of antiserum against 135-ABP into living root hair cells induced the disappearance of the transvacuolar strand. Concomitantly, thick AF bundles in the transvacuolar strand dispersed into thin bundles. In the root hair cells, AFs showed uniform polarity in the bundles, which is consistent with the in-vitro activity of 135-ABP. These results suggest that villin is a factor responsible for bundling AFs in root hair cells as well as in pollen tubes, and that it plays a key role in determining the direction of cytoplasmic streaming in these cells.

KW - Actin bundling protein

KW - Actin filament

KW - Hydrocharis

KW - Root hair cell

KW - Transvacuolar strand

KW - Villin

UR - http://www.scopus.com/inward/record.url?scp=0034020244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034020244&partnerID=8YFLogxK

M3 - Article

C2 - 10805457

AN - SCOPUS:0034020244

VL - 210

SP - 836

EP - 843

JO - Planta

JF - Planta

SN - 0032-0935

IS - 5

ER -