The role of SmpB protein in trans-translation

Yoshihiro Shimizu, Takuya Ueda

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

Original languageEnglish
Pages (from-to)74-77
Number of pages4
JournalFEBS Letters
Volume514
Issue number1
DOIs
Publication statusPublished - 2002 Mar 6
Externally publishedYes

Fingerprint

Ribosomes
Alanine-tRNA Ligase
Peptide Elongation Factors
Cell-Free System
Alanine
Binding Sites
Peptides
tmRNA
small protein B

Keywords

  • 10Sa
  • SmpB
  • SsrA
  • tmRNA
  • trans-Translation
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The role of SmpB protein in trans-translation. / Shimizu, Yoshihiro; Ueda, Takuya.

In: FEBS Letters, Vol. 514, No. 1, 06.03.2002, p. 74-77.

Research output: Contribution to journalArticle

Shimizu, Yoshihiro ; Ueda, Takuya. / The role of SmpB protein in trans-translation. In: FEBS Letters. 2002 ; Vol. 514, No. 1. pp. 74-77.
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