The role of SmpB protein in trans-translation

Yoshihiro Shimizu, Takuya Ueda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)


The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

Original languageEnglish
Pages (from-to)74-77
Number of pages4
JournalFEBS Letters
Issue number1
Publication statusPublished - 2002 Mar 6
Externally publishedYes


  • 10Sa
  • SmpB
  • SsrA
  • Translation
  • tmRNA
  • trans-Translation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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