The role of SmpB protein in trans-translation

Yoshihiro Shimizu; Takuya Ueda

doi:10.1016/S0014-5793(02)02333-5

The role of SmpB protein in trans-translation

Yoshihiro Shimizu, Takuya Ueda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

Original language	English
Pages (from-to)	74-77
Number of pages	4
Journal	FEBS Letters
Volume	514
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(02)02333-5
Publication status	Published - 2002 Mar 6
Externally published	Yes

Keywords

10Sa
SmpB
SsrA
Translation
tmRNA
trans-Translation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)02333-5

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abstract = "The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.",

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AB - The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.

KW - 10Sa

KW - SmpB

KW - SsrA

KW - Translation

KW - tmRNA

KW - trans-Translation

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JO - FEBS Letters

JF - FEBS Letters

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ER -

The role of SmpB protein in trans-translation

Abstract

Keywords

ASJC Scopus subject areas

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