Abstract
The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
| Original language | English |
|---|---|
| Pages (from-to) | 74-77 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 514 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 Mar 6 |
| Externally published | Yes |
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Keywords
- 10Sa
- SmpB
- SsrA
- tmRNA
- trans-Translation
- Translation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The role of SmpB protein in trans-translation. / Shimizu, Yoshihiro; Ueda, Takuya.
In: FEBS Letters, Vol. 514, No. 1, 06.03.2002, p. 74-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of SmpB protein in trans-translation
AU - Shimizu, Yoshihiro
AU - Ueda, Takuya
PY - 2002/3/6
Y1 - 2002/3/6
N2 - The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
AB - The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
KW - 10Sa
KW - SmpB
KW - SsrA
KW - tmRNA
KW - trans-Translation
KW - Translation
UR - http://www.scopus.com/inward/record.url?scp=0037029092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037029092&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)02333-5
DO - 10.1016/S0014-5793(02)02333-5
M3 - Article
C2 - 11904185
AN - SCOPUS:0037029092
VL - 514
SP - 74
EP - 77
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -