The role of the DELSEED motif of the β subunit in rotation of F1- ATPase

Kiyotaka Y. Hara, Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Kazuhiko Kinosita, Masasuke Yoshida

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

F1-ATPase is a rotary motor protein, and ATP hydrolysis generates torque at the interface between the γ subunit, a rotor shaft, and the α33 substructure, a stator ring. The region of conserved acidic 'DELSEED' motif of the β subunit has a contact with γ subunit and has been assumed to be involved in torque generation. Using the thermophilic α3β3γ complex in which the corresponding sequence is DELSDED, we replaced each residue and all five acidic residues in this sequence with alanine. In addition, each of two conserved residues at the counterpart contact position of γ subunit was also replaced. Surprisingly, all of these mutants rotated with as much torque as the wild-type. We conclude that side chains of the DELSEED motif of the β subunit do not have a direct role in torque generation.

Original languageEnglish
Pages (from-to)14260-14263
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number19
DOIs
Publication statusPublished - 2000 May 12
Externally publishedYes

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Proton-Translocating ATPases
Torque
Alanine
Stators
Hydrolysis
Rotors
Adenosine Triphosphate
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hara, K. Y., Noji, H., Bald, D., Yasuda, R., Kinosita, K., & Yoshida, M. (2000). The role of the DELSEED motif of the β subunit in rotation of F1- ATPase. Journal of Biological Chemistry, 275(19), 14260-14263. https://doi.org/10.1074/jbc.275.19.14260

The role of the DELSEED motif of the β subunit in rotation of F1- ATPase. / Hara, Kiyotaka Y.; Noji, Hiroyuki; Bald, Dirk; Yasuda, Ryohei; Kinosita, Kazuhiko; Yoshida, Masasuke.

In: Journal of Biological Chemistry, Vol. 275, No. 19, 12.05.2000, p. 14260-14263.

Research output: Contribution to journalArticle

Hara, KY, Noji, H, Bald, D, Yasuda, R, Kinosita, K & Yoshida, M 2000, 'The role of the DELSEED motif of the β subunit in rotation of F1- ATPase', Journal of Biological Chemistry, vol. 275, no. 19, pp. 14260-14263. https://doi.org/10.1074/jbc.275.19.14260
Hara, Kiyotaka Y. ; Noji, Hiroyuki ; Bald, Dirk ; Yasuda, Ryohei ; Kinosita, Kazuhiko ; Yoshida, Masasuke. / The role of the DELSEED motif of the β subunit in rotation of F1- ATPase. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 19. pp. 14260-14263.
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