The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells

Chunnan Li, Kazunaga Yazawa, Seiji Kondo, Yoshiki Mukudai, Daisuke Sato, Yuji Kurihara, Takaaki Kamatani, Satoru Shintani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Currently there is growing use of complementary and alternative anticancer medicines worldwide, and considerable interest in finding anticancer drugs among Chinese medicinal herbs. The aim of this study was to determine the antitumor activity of the root bark of Paeonia moutan (RBPM) in human squamous cell carcinoma (OSCC) cells. Cell lines derived from human oral squamous cell carcinoma (HSC2, 3, 4, SAS) were tested with different concentrations of RBPM (1-100 μg/ml) using a series of in vitro assay systems. RBPM at a concentration of 100 μg/ml inhibited monolayer and anchorage-independent growth, and interrupted coordinated migration. RBPM activated the phosphorylation of extracellular signal-regulated kinase (ERK) and serine/threonine kinase AKT in 30 min; then, at a later stage (after 6 hours) exhibited potent cytostatic, pro-apoptotic effects through the down-regulation of the expression of cyclin-dependent kinase 4 and its partner cyclin D1, and poly(ADP-ribose) polymerase cleavage. We found direct evidence that RBPM induces apoptotic cell death via DNA fragmentation. Taken together, the antitumor activity of RBPM was demonstrated through antiproliferative and apoptotic effects.

Original languageEnglish
Pages (from-to)2625-2630
Number of pages6
JournalAnticancer Research
Volume32
Issue number7
Publication statusPublished - 2012 Jul
Externally publishedYes

Fingerprint

Paeonia
Antineoplastic Agents
Squamous Cell Carcinoma
Cyclin-Dependent Kinase 4
Poly(ADP-ribose) Polymerases
Protein-Serine-Threonine Kinases
Cyclin D1
Extracellular Signal-Regulated MAP Kinases
Cytostatic Agents
DNA Fragmentation
Medicinal Plants
Complementary Therapies
Cell Death
Down-Regulation
Phosphorylation
Cell Line
Growth
Pharmaceutical Preparations

Keywords

  • Chinese medicinal herbs
  • HSC2
  • HSC3
  • HSC4
  • Oral squamous cell carcinoma
  • Paeonia moutan
  • SAS cell lines

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Li, C., Yazawa, K., Kondo, S., Mukudai, Y., Sato, D., Kurihara, Y., ... Shintani, S. (2012). The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells. Anticancer Research, 32(7), 2625-2630.

The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells. / Li, Chunnan; Yazawa, Kazunaga; Kondo, Seiji; Mukudai, Yoshiki; Sato, Daisuke; Kurihara, Yuji; Kamatani, Takaaki; Shintani, Satoru.

In: Anticancer Research, Vol. 32, No. 7, 07.2012, p. 2625-2630.

Research output: Contribution to journalArticle

Li, C, Yazawa, K, Kondo, S, Mukudai, Y, Sato, D, Kurihara, Y, Kamatani, T & Shintani, S 2012, 'The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells', Anticancer Research, vol. 32, no. 7, pp. 2625-2630.
Li C, Yazawa K, Kondo S, Mukudai Y, Sato D, Kurihara Y et al. The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells. Anticancer Research. 2012 Jul;32(7):2625-2630.
Li, Chunnan ; Yazawa, Kazunaga ; Kondo, Seiji ; Mukudai, Yoshiki ; Sato, Daisuke ; Kurihara, Yuji ; Kamatani, Takaaki ; Shintani, Satoru. / The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells. In: Anticancer Research. 2012 ; Vol. 32, No. 7. pp. 2625-2630.
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