The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice

Jun Ohgane, Teruhiko Wakayama, Sho Senda, Yukiko Yamazaki, Kimiko Inoue, Atsuo Ogura, Joel Marh, Satoshi Tanaka, Ryuzo Yanagimachi, Kunio Shiota

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

DNA methylation controls various developmental processes by silencing, switching and stabilizing genes as well as remodeling chromatin. Among various symptoms in cloned animals, placental hypertrophy is commonly observed. We identified the Spalt-like gene3 (Sall3) locus as a hypermethylated region in the placental genome of cloned mice. The Sall3 locus has a CpG island containing a tissue-dependent differentially methylated region (T-DMR) specific to the trophoblast cell lineage. The T-DMR sequence is also conserved in the human genome at the SALL3 locus of chromosome 18q23, which has been suggested to be involved in the 18q deletion syndrome. Intriguingly, larger placentas were more heavily methylated at the Sall3 locus in cloned mice. This epigenetic error was found in all cloned mice examined regardless of sex, mouse strain and the type of donor cells. In contrast, the placentas of in vitro fertilized (IVF) and intracytoplasmic sperm injected (ICSI) mice did not show such hypermethylation, suggesting that aberrant hypermethylation at the Sall3 locus is associated with abnormal placental development caused by nuclear transfer of somatic cells. We concluded that the Sall3 locus is the area with frequent epigenetic errors in cloned mice.These data suggest that there exists at least genetic locus that is highly susceptible to epigenetic error caused by nuclear transfer.

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalGenes to Cells
Volume9
Issue number3
DOIs
Publication statusPublished - 2004 Mar
Externally publishedYes

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DNA Methylation
Epigenomics
Placenta
Placentation
CpG Islands
Genetic Loci
Chromatin Assembly and Disassembly
Trophoblasts
Cell Lineage
Human Genome
Hypertrophy
Spermatozoa
Chromosomes
Genome
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice. / Ohgane, Jun; Wakayama, Teruhiko; Senda, Sho; Yamazaki, Yukiko; Inoue, Kimiko; Ogura, Atsuo; Marh, Joel; Tanaka, Satoshi; Yanagimachi, Ryuzo; Shiota, Kunio.

In: Genes to Cells, Vol. 9, No. 3, 03.2004, p. 253-260.

Research output: Contribution to journalArticle

Ohgane, J, Wakayama, T, Senda, S, Yamazaki, Y, Inoue, K, Ogura, A, Marh, J, Tanaka, S, Yanagimachi, R & Shiota, K 2004, 'The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice', Genes to Cells, vol. 9, no. 3, pp. 253-260. https://doi.org/10.1111/j.1356-9597.2004.00720.x
Ohgane, Jun ; Wakayama, Teruhiko ; Senda, Sho ; Yamazaki, Yukiko ; Inoue, Kimiko ; Ogura, Atsuo ; Marh, Joel ; Tanaka, Satoshi ; Yanagimachi, Ryuzo ; Shiota, Kunio. / The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice. In: Genes to Cells. 2004 ; Vol. 9, No. 3. pp. 253-260.
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